Detailed information of TA system TA150926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 389183..389910
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: G6Z98_RS01810
• Protein ID: WP_001521139.1
• Coordinates: 389183..389494 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6Z98_RS01815
• Protein ID: WP_000126294.1
• Coordinates: 389491..389910 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS01785	385118..386827	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
G6Z98_RS01790	386837..387379	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6Z98_RS01795	387379..388146	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
G6Z98_RS01800	388143..388553	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6Z98_RS01805	388546..389016	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6Z98_RS01810	389183..389494	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6Z98_RS01815	389491..389910	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
G6Z98_RS01820	389989..391413	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
G6Z98_RS01825	391422..392879	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6Z98_RS01830	393139..394149	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
G6Z98_RS01835	394298..394825	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T150926 WP_001521139.1 NZ_CP049197:389183-389494 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150926 NZ_CP065148:c14981-14598 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT150926 WP_000126294.1 NZ_CP049197:389491-389910 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150926 NZ_CP065148:c15199-14978 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATTTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCGCTGACAAATCGATGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9