Detailed information of TA system TA150923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4463729..4464331
• Replicon: chromosome
• Accession: NZ_CP049196
• Organism: Escherichia coli strain E118

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G5T40_RS21365
• Protein ID: WP_000897305.1
• Coordinates: 4464020..4464331 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5T40_RS21360
• Protein ID: WP_000356395.1
• Coordinates: 4463729..4464019 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5T40_RS21325	4459352..4460254	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
G5T40_RS21330	4460251..4460886	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G5T40_RS21335	4460883..4461812	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G5T40_RS21340	4461994..4462236	-	243	WP_021523315.1	hypothetical protein	-
G5T40_RS21345	4462455..4462673	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
G5T40_RS21350	4463092..4463370	-	279	WP_001306650.1	hypothetical protein	-
G5T40_RS21355	4463432..4463644	-	213	WP_000197774.1	hypothetical protein	-
G5T40_RS21360	4463729..4464019	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
G5T40_RS21365	4464020..4464331	-	312	WP_000897305.1	hypothetical protein	Toxin
G5T40_RS21370	4464560..4465468	+	909	WP_001306651.1	alpha/beta hydrolase	-
G5T40_RS21375	4465637..4467451	-	1815	WP_020233236.1	hypothetical protein	-
G5T40_RS21380	4467867..4468808	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G5T40_RS21385	4468853..4469290	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T150923 WP_000897305.1 NZ_CP049196:c4464331-4464020 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T150923 NZ_CP065147:c2021182-2020658 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT150923 WP_000356395.1 NZ_CP049196:c4464019-4463729 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT150923 NZ_CP065147:c2021457-2021179 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7