Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3505401..3506095 Replicon chromosome
Accession NZ_CP049196
Organism Escherichia coli strain E118

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag G5T40_RS16850 Protein ID WP_001521903.1
Coordinates 3505401..3505799 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag G5T40_RS16855 Protein ID WP_000554758.1
Coordinates 3505802..3506095 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5T40_RS16820 3500571..3501815 - 1245 WP_000189541.1 esterase FrsA -
- 3501231..3501311 - 81 NuclAT_11 - -
- 3501231..3501311 - 81 NuclAT_11 - -
- 3501231..3501311 - 81 NuclAT_11 - -
- 3501231..3501311 - 81 NuclAT_11 - -
G5T40_RS16825 3501907..3502365 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
G5T40_RS16830 3502626..3504083 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
G5T40_RS16835 3504140..3504492 - 353 Protein_3289 peptide chain release factor H -
G5T40_RS16840 3504488..3504694 - 207 Protein_3290 RtcB family protein -
G5T40_RS16845 3504939..3505391 - 453 WP_023144376.1 GNAT family N-acetyltransferase -
G5T40_RS16850 3505401..3505799 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
G5T40_RS16855 3505802..3506095 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
G5T40_RS16860 3506147..3507202 - 1056 WP_001226177.1 DNA polymerase IV -
G5T40_RS16865 3507273..3508058 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
G5T40_RS16870 3508030..3509742 + 1713 Protein_3296 flagellar biosynthesis protein FlhA -
G5T40_RS16875 3509821..3510174 + 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
G5T40_RS16880 3510231..3510980 - 750 WP_016233951.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3494038..3506095 12057


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T150916 WP_001521903.1 NZ_CP049196:c3505799-3505401 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T150916 NZ_CP065146:c572556-571987 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCCTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGATTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGCAGCAGCAATAAAAGGGCAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCACAAAAATACCTTAATTCTTTTGATATTTCAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGAATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT150916 WP_000554758.1 NZ_CP049196:c3506095-3505802 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT150916 NZ_CP065146:c573008-572556 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References