Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1359255..1359757 Replicon chromosome
Accession NZ_CP049196
Organism Escherichia coli strain E118

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G5T40_RS06345 Protein ID WP_000767829.1
Coordinates 1359503..1359757 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G5T40_RS06340 Protein ID WP_001259255.1
Coordinates 1359255..1359506 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5T40_RS06315 1354433..1355500 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G5T40_RS06320 1355500..1356570 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
G5T40_RS06325 1356567..1357871 - 1305 WP_021523144.1 histidinol dehydrogenase -
G5T40_RS06330 1357877..1358776 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
G5T40_RS06335 1358922..1358972 - 51 WP_001364200.1 his operon leader peptide -
G5T40_RS06340 1359255..1359506 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G5T40_RS06345 1359503..1359757 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G5T40_RS06350 1359840..1360664 + 825 WP_000754758.1 SDR family oxidoreductase -
G5T40_RS06355 1360710..1361639 + 930 WP_023154044.1 LysR family transcriptional regulator -
G5T40_RS06360 1361853..1361915 + 63 WP_010723108.1 membrane protein YoeI -
G5T40_RS06365 1361905..1363263 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G5T40_RS06370 1363442..1364500 + 1059 WP_000492324.1 transport protein YeeE -
G5T40_RS06375 1364514..1364741 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T150898 WP_000767829.1 NZ_CP049196:1359503-1359757 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T150898 NZ_CP065136:c4768074-4767901 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT150898 WP_001259255.1 NZ_CP049196:1359255-1359506 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT150898 NZ_CP065136:c4768398-4768102 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References