Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 46982..47781 Replicon chromosome
Accession NZ_CP049196
Organism Escherichia coli strain E118

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag G5T40_RS00250 Protein ID WP_000347272.1
Coordinates 46982..47446 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G5T40_RS00255 Protein ID WP_001307405.1
Coordinates 47446..47781 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5T40_RS00220 41983..42417 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
G5T40_RS00225 42435..43313 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G5T40_RS00230 43303..44082 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G5T40_RS00235 44093..44566 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G5T40_RS00240 44589..45869 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
G5T40_RS00245 46118..46927 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
G5T40_RS00250 46982..47446 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G5T40_RS00255 47446..47781 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G5T40_RS00260 47930..49501 - 1572 WP_001273738.1 galactarate dehydratase -
G5T40_RS00265 49876..51210 + 1335 WP_089502604.1 galactarate/glucarate/glycerate transporter GarP -
G5T40_RS00270 51226..51996 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T150893 WP_000347272.1 NZ_CP049196:c47446-46982 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T150893 NZ_CP065136:c4165054-4164656 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT150893 WP_001307405.1 NZ_CP049196:c47781-47446 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT150893 NZ_CP065136:c4165350-4165057 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References