Detailed information of TA system TA150886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2921844..2922574
• Replicon: chromosome
• Accession: NZ_CP049192
• Organism: Enterobacter hormaechei strain Y2152

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: G5577_RS13705
• Protein ID: WP_023302825.1
• Coordinates: 2921844..2922158 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5577_RS13710
• Protein ID: WP_023323924.1
• Coordinates: 2922155..2922574 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5577_RS13680	2917511..2918596	+	1086	Protein_2674	cellulase family glycosylhydrolase	-
G5577_RS13685	2918502..2919686	-	1185	WP_048245027.1	multidrug efflux MFS transporter EmrD	-
G5577_RS13690	2919862..2920695	-	834	WP_023323922.1	DMT family transporter	-
G5577_RS13695	2920758..2921204	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
G5577_RS13700	2921269..2921358	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
G5577_RS13705	2921844..2922158	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
G5577_RS13710	2922155..2922574	+	420	WP_023323924.1	helix-turn-helix domain-containing protein	Antitoxin
G5577_RS13715	2922721..2922819	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
G5577_RS13720	2923049..2924737	+	1689	WP_003861056.1	acetolactate synthase large subunit	-
G5577_RS13725	2924741..2925028	+	288	WP_003861055.1	acetolactate synthase small subunit	-
G5577_RS13730	2925111..2925704	+	594	WP_003861054.1	transcriptional regulator UhpA	-
G5577_RS13735	2925701..2927206	+	1506	WP_045336028.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T150886 WP_023302825.1 NZ_CP049192:2921844-2922158 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T150886 NZ_CP065136:c2724027-2723851 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15499.15 Da
• Isoelectric Point: 5.7670

>AT150886 WP_023323924.1 NZ_CP049192:2922155-2922574 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT150886 NZ_CP065136:c2723805-2723389 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A837FIL2