Detailed information of TA system TA150884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1727416..1728073
• Replicon: chromosome
• Accession: NZ_CP049192
• Organism: Enterobacter hormaechei strain Y2152

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G5577_RS08030
• Protein ID: WP_063978808.1
• Coordinates: 1727416..1727715 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0F0ZCT3
• Locus tag: G5577_RS08035
• Protein ID: WP_022651701.1
• Coordinates: 1727726..1728073 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5577_RS08020	1723793..1725985	+	2193	WP_045346161.1	type I secretion system permease/ATPase	-
G5577_RS08025	1726005..1727165	+	1161	WP_100154277.1	HlyD family efflux transporter periplasmic adaptor subunit	-
G5577_RS08030	1727416..1727715	+	300	WP_063978808.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G5577_RS08035	1727726..1728073	+	348	WP_022651701.1	HigA family addiction module antidote protein	Antitoxin
G5577_RS08040	1728149..1729330	-	1182	WP_022651702.1	PLP-dependent aminotransferase family protein	-
G5577_RS08045	1729350..1730237	-	888	WP_032660046.1	LysR family transcriptional regulator	-
G5577_RS08050	1730335..1730937	+	603	WP_164474400.1	short chain dehydrogenase	-
G5577_RS08055	1730934..1731665	-	732	WP_137048338.1	class I SAM-dependent methyltransferase	-
G5577_RS08060	1731740..1732738	-	999	WP_045346167.1	transketolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11606.25 Da
• Isoelectric Point: 9.6776

>T150884 WP_063978808.1 NZ_CP049192:1727416-1727715 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNIYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T150884 NZ_CP065136:2262326-2262429 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13096.09 Da
• Isoelectric Point: 9.5932

>AT150884 WP_022651701.1 NZ_CP049192:1727726-1728073 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Nucleotide

• Length: 348 bp

>AT150884 NZ_CP065136:c2262467-2262196 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0F0ZCT3