Detailed information of TA system TA150862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2652921..2653651
• Replicon: chromosome
• Accession: NZ_CP049188
• Organism: Enterobacter hormaechei strain Y323

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: G5C52_RS12590
• Protein ID: WP_023302825.1
• Coordinates: 2653337..2653651 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5C52_RS12585
• Protein ID: WP_023323924.1
• Coordinates: 2652921..2653340 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C52_RS12560	2648289..2649794	-	1506	WP_045336028.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
G5C52_RS12565	2649791..2650384	-	594	WP_003861054.1	transcriptional regulator UhpA	-
G5C52_RS12570	2650467..2650754	-	288	WP_003861055.1	acetolactate synthase small subunit	-
G5C52_RS12575	2650758..2652446	-	1689	WP_003861056.1	acetolactate synthase large subunit	-
G5C52_RS12580	2652676..2652774	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
G5C52_RS12585	2652921..2653340	-	420	WP_023323924.1	helix-turn-helix domain-containing protein	Antitoxin
G5C52_RS12590	2653337..2653651	-	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
G5C52_RS12595	2654137..2654226	+	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
G5C52_RS12600	2654291..2654737	+	447	WP_003861064.1	GNAT family N-acetyltransferase	-
G5C52_RS12605	2654800..2655633	+	834	WP_023323922.1	DMT family transporter	-
G5C52_RS12610	2655809..2656993	+	1185	WP_048245027.1	multidrug efflux MFS transporter EmrD	-
G5C52_RS12615	2656899..2657984	-	1086	Protein_2462	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T150862 WP_023302825.1 NZ_CP049188:c2653651-2653337 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T150862 NZ_CP065054:1008083-1008340 [Streptococcus equi subsp. zooepidemicus]
ATGAGTTATAAGCTTATTCCAACACCTTTGTTCGCAAAGCAATTTAAAAAGCTTGATAAATTTGTCCAAAAACAAATAAA
ATCATATCTGGAAAAAGTGCTGAGTGACCCTAGAGCTAAGGGGAAAGGATTAGTAGCTAATAGAAGCGGACAGTGGCGAT
ACCGTATTGGGGATTATCGTGTCATAGTCAATATCCAAGATGGTGAAATGATAGTTCTTGCTCTTGAAGTTGGGCACAGA
AAAGATATTTATAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15499.15 Da
• Isoelectric Point: 5.7670

>AT150862 WP_023323924.1 NZ_CP049188:c2653340-2652921 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT150862 NZ_CP065054:1007853-1008086 [Streptococcus equi subsp. zooepidemicus]
ATGGCAGTTATTACACTAAAAATATCAGAGCAAGAAAAATCATTTTTACAAGGAATGGCTAAATTTGAAGGCATTAGCTT
ATCTGAACTAATCCGTAAAAAAACGCTAAAATCACTTGAAGATGAGTATGACGCACGAGTAGCAGATATGGCTTTAGAGG
ATTACGAGAACTATTTAGAAAACGGAGGACAAGTTTTGTCTTGGGCTGCATTGATGAACGAGGTTGATTTATGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2