Detailed information of TA system TA150845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2032598..2033325
• Replicon: chromosome
• Accession: NZ_CP049185
• Organism: Shigella sonnei strain 19.0821.3486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O60_RS10670
• Protein ID: WP_000547563.1
• Coordinates: 2033014..2033325 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O60_RS10665
• Protein ID: WP_000126296.1
• Coordinates: 2032598..2033017 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O60_RS10645 (2027649)	2027649..2028176	-	528	WP_001078766.1	electron transport protein HydN	-
G6O60_RS10650 (2028324)	2028324..2029334	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O60_RS10655 (2029594)	2029594..2031051	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O60_RS10660 (2031060)	2031060..2032484	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O60_RS10665 (2032598)	2032598..2033017	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O60_RS10670 (2033014)	2033014..2033325	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O60_RS10675 (2033487)	2033487..2034260	-	774	WP_001026459.1	hypothetical protein	-
G6O60_RS10680 (2034285)	2034285..2034755	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O60_RS10685 (2034748)	2034748..2035158	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O60_RS10690 (2035155)	2035155..2035922	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O60_RS10695 (2035922)	2035922..2036464	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O60_RS10700 (2036474)	2036474..2038171	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150845 WP_000547563.1 NZ_CP049185:c2033325-2033014 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150845 NZ_CP065044:4411645-4411899 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150845 WP_000126296.1 NZ_CP049185:c2033017-2032598 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150845 NZ_CP065044:4411397-4411648 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAATATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAAGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22