Detailed information of TA system TA150820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2536853..2537580
• Replicon: chromosome
• Accession: NZ_CP049179
• Organism: Shigella sonnei strain L4094

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O64_RS12475
• Protein ID: WP_000547563.1
• Coordinates: 2536853..2537164 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O64_RS12480
• Protein ID: WP_000126296.1
• Coordinates: 2537161..2537580 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O64_RS12445 (2532007)	2532007..2533704	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
G6O64_RS12450 (2533714)	2533714..2534256	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O64_RS12455 (2534256)	2534256..2535023	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O64_RS12460 (2535020)	2535020..2535430	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O64_RS12465 (2535423)	2535423..2535893	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O64_RS12470 (2535918)	2535918..2536691	+	774	WP_001026459.1	hypothetical protein	-
G6O64_RS12475 (2536853)	2536853..2537164	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O64_RS12480 (2537161)	2537161..2537580	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O64_RS12485 (2537694)	2537694..2539118	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O64_RS12490 (2539127)	2539127..2540584	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O64_RS12495 (2540844)	2540844..2541854	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O64_RS12500 (2542002)	2542002..2542529	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150820 WP_000547563.1 NZ_CP049179:2536853-2537164 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150820 NZ_CP065039:1513712-1514095 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150820 WP_000126296.1 NZ_CP049179:2537161-2537580 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150820 NZ_CP065039:1513494-1513715 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATTTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCACTGACAAATCGATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22