150817

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2259965..2260692 Replicon chromosome
Accession NZ_CP049179
Organism Shigella sonnei strain L4094

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G6O64_RS11120 Protein ID WP_000550189.1
Coordinates 2260378..2260692 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G6O64_RS11115 Protein ID WP_000560263.1
Coordinates 2259965..2260381 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O64_RS11105 (2255325) 2255325..2257676 + 2352 WP_000695471.1 alpha-glucosidase -
G6O64_RS11110 (2257902) 2257902..2259920 + 2019 WP_000121451.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G6O64_RS11115 (2259965) 2259965..2260381 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G6O64_RS11120 (2260378) 2260378..2260692 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G6O64_RS11125 (2260977) 2260977..2262113 - 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G6O64_RS11130 (2262198) 2262198..2262701 + 504 WP_001305117.1 M48 family metallopeptidase -
G6O64_RS11135 (2262778) 2262778..2263461 + 684 WP_001183060.1 vancomycin high temperature exclusion protein -
G6O64_RS11140 (2263540) 2263540..2264526 + 987 WP_001446103.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T150817 WP_000550189.1 NZ_CP049179:c2260692-2260378 [Shigella sonnei]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T150817 NZ_CP065037:4124579-4124839 [Leifsonia sp. ZF2019]
ATGAAGCTGATCTGGGACCAGAACGCCTGGGAGGACTACCTCTACTGGCAGGCGCAGGACCGTCGCGTAGTGAAGCGCAT
CAACCAGCTCCTGGTGGACATCCTGCGCAACGGCAATACCGGGATCGGCAAGCCCGAGGCGCTCAAGCACGGGTTCCAGG
GGTTCTGGTCGCGGCGCATCACGGATGAGCATCGGCTCGTCTACAAGGTGACGGAGGATGCGGTGCTGATCGCCCAGTGT
CGGTACCACTACGCCCCGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT150817 WP_000560263.1 NZ_CP049179:c2260381-2259965 [Shigella sonnei]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT150817 NZ_CP065037:4124331-4124582 [Leifsonia sp. ZF2019]
ATGAAGACGCTCAGCTATTCGGAGTCGCGCGCGCACTATGCCGCTACGCTCGACGCCGTGCTCGACGACCGCGAGGAGGT
CGTCATCACCCGCGCCGGGCGGGAGCCTGTGGTGATGGTCGCGCTCGAGGACTACGAGTCGCTCAAGGAGACCGCCTACC
TGCTGCGTAGCCCGGCCAACGCGCGCCGCCTGATCGAGTCGATCGAACGTCTCGATGACGGCGAAGGCACCCCGCACGAT
CTGCTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References