Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 413746..414287 Replicon chromosome
Accession NZ_CP049179
Organism Shigella sonnei strain L4094

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O64_RS02020 Protein ID WP_000615976.1
Coordinates 414009..414287 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O64_RS02015 Protein ID WP_000729704.1
Coordinates 413746..414006 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O64_RS01990 (409215) 409215..410927 + 1713 Protein_390 flagellar biosynthesis protein FlhA -
G6O64_RS01995 (411050) 411050..411547 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O64_RS02000 (411618) 411618..411746 - 129 Protein_392 ISAs1 family transposase -
G6O64_RS02005 (411801) 411801..412498 + 698 WP_237158194.1 IS1-like element IS1A family transposase -
G6O64_RS02010 (412787) 412787..413560 - 774 WP_127148796.1 C40 family peptidase -
G6O64_RS02015 (413746) 413746..414006 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O64_RS02020 (414009) 414009..414287 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O64_RS02025 (414443) 414443..415183 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O64_RS02030 (415154) 415154..415921 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6O64_RS02035 (416127) 416127..416705 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 412174..425148 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150815 WP_000615976.1 NZ_CP049179:414009-414287 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150815 NZ_CP065037:3542055-3542315 [Leifsonia sp. ZF2019]
GTGAGCCGCGAACTCGCCTTCGACCGGCACGGCTGGGACGACTACTGCTACTGGCAGACCGAAGACCGCAAGACCCTGAA
ACGGATCAATCAGCTGATCCAGGACGTCCTCCGCGACCCCTTCACGGGAATCGGGAAGCCCGAGCCGTTGAAGCATGTGC
TCTCGGGCGCCTGGTCGCGCCGCATCGACGACAGCAACCGACTGGTCTATTACGTCACCGACAGCCACATCGTGGTCCTG
CAAGCGCGAGAGCACTACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150815 WP_000729704.1 NZ_CP049179:413746-414006 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150815 NZ_CP065037:3541816-3542058 [Leifsonia sp. ZF2019]
ATGACAGCGACGGATGCGCGCAAGGGTTTGTTCGGGCTGATTCAGCAGGTCAATGAGGATCACACCTCGATCGAGGTGGT
CTCCAAGCACGGCAATGTGGTCATCATGTCGAAGGACGACTACGACGCGATGACGGAGACCGCGCATCTGTTGCGGAACG
CTCCGAACGCCGAGCGCCTTCTCGAATCGATCGAACGCGCACGGCGTGGCGAGTTCGAACAGCACGACCTCCTCGACACG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References