Detailed information of TA system TA150809

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52790..53054
• Replicon: plasmid p1205-3131
• Accession: NZ_CP049178
• Organism: Shigella sonnei strain 1205.3131

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: G6O63_RS25015
• Protein ID: WP_001387489.1
• Coordinates: 52790..52942 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	52992..53054 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O63_RS24985 (48091)	48091..50259	+	2169	WP_001774191.1	DotA/TraY family protein	-
G6O63_RS24990 (50333)	50333..50983	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
G6O63_RS24995 (51055)	51055..51264	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
G6O63_RS25000 (51630)	51630..51806	+	177	WP_001054898.1	hypothetical protein	-
G6O63_RS25315 (51871)	51871..51966	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
G6O63_RS25010 (52467)	52467..52718	+	252	WP_001291964.1	hypothetical protein	-
G6O63_RS25015 (52790)	52790..52942	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (52992)	52992..53054	+	63	NuclAT_0	-	Antitoxin
- (52992)	52992..53054	+	63	NuclAT_0	-	Antitoxin
- (52992)	52992..53054	+	63	NuclAT_0	-	Antitoxin
- (52992)	52992..53054	+	63	NuclAT_0	-	Antitoxin
G6O63_RS25020 (53569)	53569..54348	+	780	WP_275450201.1	protein FinQ	-
G6O63_RS25025 (54655)	54655..55863	+	1209	WP_107372241.1	IncI1-type conjugal transfer protein TrbA	-
G6O63_RS25030 (55882)	55882..56952	+	1071	WP_000151590.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-3	-	1..86039	86039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T150809 WP_001387489.1 NZ_CP049178:c52942-52790 [Shigella sonnei]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T150809 NZ_CP065036:2875704-2875907 [Pectobacterium parmentieri]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACGATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCCAATGATGAACTGGAGATGTTCTTTTCTGCAGCCGATCATCGCCTGGCAGAGTTGACGATGAACAAAC
TCTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT150809 NZ_CP049178:52992-53054 [Shigella sonnei]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin