Detailed information of TA system TA150804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3495758..3496485
• Replicon: chromosome
• Accession: NZ_CP049177
• Organism: Shigella sonnei strain 1205.3131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O63_RS17820
• Protein ID: WP_000547563.1
• Coordinates: 3496174..3496485 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O63_RS17815
• Protein ID: WP_000126296.1
• Coordinates: 3495758..3496177 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O63_RS17795 (3490809)	3490809..3491336	-	528	WP_001078766.1	electron transport protein HydN	-
G6O63_RS17800 (3491484)	3491484..3492494	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O63_RS17805 (3492754)	3492754..3494211	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O63_RS17810 (3494220)	3494220..3495644	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O63_RS17815 (3495758)	3495758..3496177	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O63_RS17820 (3496174)	3496174..3496485	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O63_RS17825 (3496647)	3496647..3497420	-	774	WP_001026459.1	hypothetical protein	-
G6O63_RS17830 (3497445)	3497445..3497915	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O63_RS17835 (3497908)	3497908..3498318	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O63_RS17840 (3498315)	3498315..3499082	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O63_RS17845 (3499082)	3499082..3499624	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O63_RS17850 (3499634)	3499634..3501331	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150804 WP_000547563.1 NZ_CP049177:c3496485-3496174 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150804 NZ_CP065036:129444-129719 [Pectobacterium parmentieri]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTTGAACGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCATCA
TGCTGACGAGGTGTTAGATCGCTTGATCATCGGAACCACAGGGATTGCTGACCACCCGAGAATTGGCGTATCTCAGACAA
GATATGAACCACGCGAGGTGAGAAAGATTTTGTTTGATGATTATGAAGTTCACTATGAAATTCAGCACGATACGATTTAT
ATCGTCGATCTTTGGCATACACGAGAAGATCGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150804 WP_000126296.1 NZ_CP049177:c3496177-3495758 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150804 NZ_CP065036:129192-129440 [Pectobacterium parmentieri]
ATGGGACAACCTGCGAAACAGACGATCAGTGCTCAGATACCCGCCGAACTTGCGGCTGCGGTTGAAAGCCTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAGCGGCGGCATCAGCGTG
TTTTGAAAGGTCTTGCTGATGTCGATGCGGGAAGAGTAGTAAGCCATGCAGATGTCATCGATTTCGCCAACAAACTGAAA
AAGTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22