Detailed information of TA system TA150783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3445960..3446687
• Replicon: chromosome
• Accession: NZ_CP049175
• Organism: Shigella sonnei strain 7111.69

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O62_RS17225
• Protein ID: WP_000547563.1
• Coordinates: 3445960..3446271 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O62_RS17230
• Protein ID: WP_000126296.1
• Coordinates: 3446268..3446687 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O62_RS17195 (3441114)	3441114..3442811	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
G6O62_RS17200 (3442821)	3442821..3443363	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O62_RS17205 (3443363)	3443363..3444130	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O62_RS17210 (3444127)	3444127..3444537	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O62_RS17215 (3444530)	3444530..3445000	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O62_RS17220 (3445025)	3445025..3445798	+	774	WP_001026459.1	hypothetical protein	-
G6O62_RS17225 (3445960)	3445960..3446271	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O62_RS17230 (3446268)	3446268..3446687	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O62_RS17235 (3446801)	3446801..3448225	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O62_RS17240 (3448234)	3448234..3449691	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O62_RS17245 (3449951)	3449951..3450961	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O62_RS17250 (3451109)	3451109..3451636	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150783 WP_000547563.1 NZ_CP049175:3445960-3446271 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150783 NZ_CP065032:c1629558-1629274 [Serratia sp. CMO1]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACATCCCCGCCAACAAACTCGCCGGTTTGCCTGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150783 WP_000126296.1 NZ_CP049175:3446268-3446687 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150783 NZ_CP065032:c1629799-1629548 [Serratia sp. CMO1]
ATGACGGTTCAACCCATTCTTGCCGACTCGGCAGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAGTACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22