Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1322858..1323399 Replicon chromosome
Accession NZ_CP049175
Organism Shigella sonnei strain 7111.69

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O62_RS06780 Protein ID WP_000615976.1
Coordinates 1323121..1323399 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O62_RS06775 Protein ID WP_000729704.1
Coordinates 1322858..1323118 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O62_RS06750 (1318327) 1318327..1320039 + 1713 Protein_1321 flagellar biosynthesis protein FlhA -
G6O62_RS06755 (1320162) 1320162..1320659 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O62_RS06760 (1320730) 1320730..1320858 - 129 Protein_1323 ISAs1 family transposase -
G6O62_RS06765 (1320913) 1320913..1321610 + 698 WP_237158194.1 IS1-like element IS1A family transposase -
G6O62_RS06770 (1321899) 1321899..1322672 - 774 WP_127148796.1 C40 family peptidase -
G6O62_RS06775 (1322858) 1322858..1323118 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O62_RS06780 (1323121) 1323121..1323399 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O62_RS06785 (1323555) 1323555..1324295 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O62_RS06790 (1324266) 1324266..1325033 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6O62_RS06795 (1325239) 1325239..1325817 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1321286..1334260 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150778 WP_000615976.1 NZ_CP049175:1323121-1323399 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150778 NZ_CP065031:c4591102-4590815 [Pectobacterium brasiliense]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGCTAGGGCATCCTGTCCGGGAACAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGAGTTGGAAAG
CGCGAAGGTAGCCAGATTTATTCTGATACGAAAGGTCGCTCTGCTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150778 WP_000729704.1 NZ_CP049175:1322858-1323118 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150778 NZ_CP065031:c4591346-4591092 [Pectobacterium brasiliense]
ATGGCTTTCAACATCCTAACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTTCGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGTGTGCCTCCTGCTGTATTCGCCTATTTAA
TGGAACTGGCTGAAGATGCAGAACTGGGGCGTACCGTTGATGATCGGATGTCAGAGCTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References