Detailed information of TA system TA150764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4733185..4733912
• Replicon: chromosome
• Accession: NZ_CP049173
• Organism: Shigella sonnei strain 19.0820.1561

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O61_RS23850
• Protein ID: WP_000547563.1
• Coordinates: 4733185..4733496 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O61_RS23855
• Protein ID: WP_000126296.1
• Coordinates: 4733493..4733912 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O61_RS23820 (4728339)	4728339..4730036	+	1698	WP_171781721.1	formate hydrogenlyase subunit HycE	-
G6O61_RS23825 (4730046)	4730046..4730588	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O61_RS23830 (4730588)	4730588..4731355	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O61_RS23835 (4731352)	4731352..4731762	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O61_RS23840 (4731755)	4731755..4732225	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O61_RS23845 (4732250)	4732250..4733023	+	774	WP_001026444.1	hypothetical protein	-
G6O61_RS23850 (4733185)	4733185..4733496	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O61_RS23855 (4733493)	4733493..4733912	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O61_RS23860 (4734026)	4734026..4735450	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O61_RS23865 (4735459)	4735459..4736916	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O61_RS23870 (4737176)	4737176..4738186	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O61_RS23875 (4738334)	4738334..4738861	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150764 WP_000547563.1 NZ_CP049173:4733185-4733496 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150764 NZ_CP065031:c26126-25740 [Pectobacterium brasiliense]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCACTATTCGATACGTGGTTTGATGAGCAAACTGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGCTATACTCGAAGAGGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCAAAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGCAAAGTTGTTCTACGAACGTATGATCAA
GATCGCAGACGCTGAGTTTGCATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACGCTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150764 WP_000126296.1 NZ_CP049173:4733493-4733912 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150764 NZ_CP065031:c25753-25454 [Pectobacterium brasiliense]
ATGGCGACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCGTTAGCATTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAAAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22