Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2617795..2618336 Replicon chromosome
Accession NZ_CP049173
Organism Shigella sonnei strain 19.0820.1561

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O61_RS13480 Protein ID WP_000615976.1
Coordinates 2618058..2618336 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O61_RS13475 Protein ID WP_000729704.1
Coordinates 2617795..2618055 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O61_RS13450 (2613254) 2613254..2614966 + 1713 Protein_2628 flagellar biosynthesis protein FlhA -
G6O61_RS13455 (2615089) 2615089..2615586 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O61_RS13460 (2615657) 2615657..2615785 - 129 Protein_2630 ISAs1 family transposase -
G6O61_RS13465 (2615840) 2615840..2616537 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
G6O61_RS13470 (2616826) 2616826..2617609 - 784 Protein_2632 C40 family peptidase -
G6O61_RS13475 (2617795) 2617795..2618055 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O61_RS13480 (2618058) 2618058..2618336 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O61_RS13485 (2618492) 2618492..2619232 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O61_RS13490 (2619203) 2619203..2619970 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6O61_RS13495 (2620176) 2620176..2620754 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2616213..2629197 12984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150759 WP_000615976.1 NZ_CP049173:2618058-2618336 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150759 NZ_CP065030:c3001373-3000969 [Pectobacterium versatile]
ATGCTTAAATATCTGCTCGATACCAATATTTGTATTTACACCATCAAGAACAAACCGCAGGAAGTCAGGGACGCTTTTTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGCTGATGGAACTGATCTATGGTGCGGAGAAATCGGTAAATCCAG
AAAAGAATCTTGCCGTGATTGAAGGGTTTTCTGCGCGTCTGGAAGTGCAAACTTATGGCTTTGACGCTGCCGTACATACG
GGGCAAATTCGTGCGGAACTGGCAAGACAAGGAACCCCCATCGGCCCGTATGATGCCATGTTAGCGGGTCATGCGCGGTC
CGCAGGTTTGATTCTGGTAACGAACAATGTGCGTGAGTTTGAGCGTGTGCCAGGATTGCGCGTAGAAAACTGGGTCGGTA
GATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150759 WP_000729704.1 NZ_CP049173:2617795-2618055 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150759 NZ_CP065030:c3001603-3001373 [Pectobacterium versatile]
ATGGAAAAAACTACAGTATTTAAAAGCAATCGCAGTCAGGCGGTACGACTCCCTAAAGCCGTTGCACTGCCGGATGATGT
TAAGCAGGTTGATATCGTCGCGATTGGTCGCACACGAATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGATGGCGTTACGCCTGACTTTATGATCACCAGAGAGCAGCCCGATGATCAGATCAGGGAAGATTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References