Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2724856..2725397 Replicon chromosome
Accession NZ_CP049171
Organism Shigella sonnei strain 0401930105

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O59_RS13505 Protein ID WP_000615976.1
Coordinates 2724856..2725134 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O59_RS13510 Protein ID WP_000729704.1
Coordinates 2725137..2725397 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O59_RS13490 (2722438) 2722438..2723016 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
G6O59_RS13495 (2723222) 2723222..2723989 + 768 WP_000333380.1 class II glutamine amidotransferase -
G6O59_RS13500 (2723960) 2723960..2724700 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O59_RS13505 (2724856) 2724856..2725134 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O59_RS13510 (2725137) 2725137..2725397 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O59_RS13515 (2725583) 2725583..2726356 + 774 WP_127148796.1 C40 family peptidase -
G6O59_RS13525 (2727397) 2727397..2727525 + 129 Protein_2619 ISAs1 family transposase -
G6O59_RS13530 (2727596) 2727596..2728093 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O59_RS13535 (2728216) 2728216..2729928 - 1713 Protein_2621 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2714706..2728093 13387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150731 WP_000615976.1 NZ_CP049171:c2725134-2724856 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150731 NZ_CP065020:2868164-2868646 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150731 WP_000729704.1 NZ_CP049171:c2725397-2725137 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150731 NZ_CP065020:2867880-2868164 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References