Detailed information of TA system TA150726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 608042..608769
• Replicon: chromosome
• Accession: NZ_CP049171
• Organism: Shigella sonnei strain 0401930105

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O59_RS03105
• Protein ID: WP_000547563.1
• Coordinates: 608458..608769 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O59_RS03100
• Protein ID: WP_000126296.1
• Coordinates: 608042..608461 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O59_RS03080 (603093)	603093..603620	-	528	WP_001078766.1	electron transport protein HydN	-
G6O59_RS03085 (603768)	603768..604778	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O59_RS03090 (605038)	605038..606495	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O59_RS03095 (606504)	606504..607928	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O59_RS03100 (608042)	608042..608461	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O59_RS03105 (608458)	608458..608769	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O59_RS03110 (608931)	608931..609704	-	774	WP_001026459.1	hypothetical protein	-
G6O59_RS03115 (609729)	609729..610199	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O59_RS03120 (610192)	610192..610602	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O59_RS03125 (610599)	610599..611366	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O59_RS03130 (611366)	611366..611908	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O59_RS03135 (611918)	611918..613615	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150726 WP_000547563.1 NZ_CP049171:c608769-608458 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150726 NZ_CP065020:c858891-858505 [Sinorhizobium meliloti]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCGGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGATTTCTCGCAAACCGATATCCCGGCGGCATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150726 WP_000126296.1 NZ_CP049171:c608461-608042 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150726 NZ_CP065020:c859145-858891 [Sinorhizobium meliloti]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22