Detailed information of TA system TA150714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2433072..2433799
• Replicon: chromosome
• Accession: NZ_CP049169
• Organism: Shigella sonnei strain 19.1125.3493

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O58_RS11960
• Protein ID: WP_000547563.1
• Coordinates: 2433072..2433383 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O58_RS11965
• Protein ID: WP_000126296.1
• Coordinates: 2433380..2433799 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O58_RS11930 (2428226)	2428226..2429923	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
G6O58_RS11935 (2429933)	2429933..2430475	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O58_RS11940 (2430475)	2430475..2431242	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O58_RS11945 (2431239)	2431239..2431649	+	411	WP_171770539.1	formate hydrogenlyase assembly protein HycH	-
G6O58_RS11950 (2431642)	2431642..2432112	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O58_RS11955 (2432137)	2432137..2432910	+	774	WP_001026459.1	hypothetical protein	-
G6O58_RS11960 (2433072)	2433072..2433383	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O58_RS11965 (2433380)	2433380..2433799	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O58_RS11970 (2433913)	2433913..2435337	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O58_RS11975 (2435346)	2435346..2436803	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O58_RS11980 (2437063)	2437063..2438073	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O58_RS11985 (2438221)	2438221..2438748	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150714 WP_000547563.1 NZ_CP049169:2433072-2433383 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150714 NZ_CP064936:c1551912-1551649 [Treponema sp. RCC2812]
ATGACTAAAGTTTGGGATGATTCTGCCTGGGATGATTATGTTTACTGGCAGGGACAGGATAGAAACACACTTAAAAAAAT
AAATTCTCTTCTAAAAGATATAGAATCAAACGGTCCGAATAAAGGAATCGGCAAACCTGAACCATTGAAACATGAATTGT
CTGGTTATTATTCGCGTAGAATTGATGAAGAAAACAGATTGGTTTACACTGTAGAAAATGATATGCTTAAAATAATTCAA
TGCAGAACTCATTATGAATATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150714 WP_000126296.1 NZ_CP049169:2433380-2433799 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150714 NZ_CP064936:c1552171-1551905 [Treponema sp. RCC2812]
ATGGCACAAACAAGTGTTAATATTCGTATGGATACCGCTACAAAGAAAAGCGCTGAAGAGTTGTTTGCCCAATTGGGAAT
GAATATGACAACAGCATTCAATATTTTTGTTCGCCAGTCATTGCGTGTTGGAGGAATTCCTTTTAATATAACAACCAAAA
CTGGTGAAGGATTTTACAATCCGTACAATCAGCGTCTTTTGCGGGAAGCGAAGGAAAGAATGGATAAAGGTTTTGGCAAA
GTCCACGAAATAATTGAAGATGACTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22