Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 316421..316962 Replicon chromosome
Accession NZ_CP049169
Organism Shigella sonnei strain 19.1125.3493

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O58_RS01555 Protein ID WP_000615976.1
Coordinates 316684..316962 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O58_RS01550 Protein ID WP_000729704.1
Coordinates 316421..316681 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O58_RS01525 (311890) 311890..313602 + 1713 Protein_301 flagellar biosynthesis protein FlhA -
G6O58_RS01530 (313725) 313725..314222 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O58_RS01535 (314293) 314293..314421 - 129 Protein_303 ISAs1 family transposase -
G6O58_RS01540 (314476) 314476..315173 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
G6O58_RS01545 (315462) 315462..316235 - 774 WP_127148796.1 C40 family peptidase -
G6O58_RS01550 (316421) 316421..316681 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O58_RS01555 (316684) 316684..316962 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O58_RS01560 (317118) 317118..317858 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O58_RS01565 (317829) 317829..318596 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6O58_RS01570 (318802) 318802..319380 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150708 WP_000615976.1 NZ_CP049169:316684-316962 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150708 NZ_CP064934:212516-212899 [Rhizobium phaseoli]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCCTATGCCATCCGGCCGTGATCGGAGAACTCGCACTTGGCAGCCTTCGGGATCGCAATAACGTCATAGCTTTCC
TGGCGGCCCAGCGCGAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCGTTTTCAGCATG
GGCATCGGCTACACAGATGCCCATTTGATGGCTTCAGTTCTACTCGATCGGCGAGCGGCCTTATGGACGAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCCCTTCACACGCCCGACAATGCGCGAAGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150708 WP_000729704.1 NZ_CP049169:316421-316681 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150708 NZ_CP064934:212319-212519 [Rhizobium phaseoli]
ATGCGATCAACTATCAATCTCGACGACACCCTCATGGAAAGGGCCAGGTTTCTAACCGGCACGAAAGAAACCGCCACTCT
GGTACGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTTATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References