Detailed information of TA system TA150703

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50997..51423
• Replicon: plasmid p09163633
• Accession: NZ_CP049168
• Organism: Shigella sonnei strain 09163633

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: G6O57_RS24940
• Protein ID: WP_001372321.1
• Coordinates: 50997..51122 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51199..51423 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O57_RS24905 (46020)	46020..46247	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
G6O57_RS24910 (46384)	46384..47055	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
G6O57_RS24915 (47249)	47249..47632	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
G6O57_RS24920 (47955)	47955..48557	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
G6O57_RS24925 (48854)	48854..49675	-	822	WP_001234445.1	DUF932 domain-containing protein	-
G6O57_RS24930 (49786)	49786..50082	-	297	WP_001272251.1	hypothetical protein	-
G6O57_RS24935 (50382)	50382..50678	+	297	Protein_61	hypothetical protein	-
G6O57_RS24940 (50997)	50997..51122	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
G6O57_RS24945 (51064)	51064..51213	-	150	Protein_63	plasmid maintenance protein Mok	-
G6O57_RS24950 (51235)	51235..51414	+	180	WP_001309233.1	hypothetical protein	-
- (51199)	51199..51423	-	225	NuclAT_0	-	Antitoxin
- (51199)	51199..51423	-	225	NuclAT_0	-	Antitoxin
- (51199)	51199..51423	-	225	NuclAT_0	-	Antitoxin
- (51199)	51199..51423	-	225	NuclAT_0	-	Antitoxin
G6O57_RS24955 (51435)	51435..52154	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
G6O57_RS24960 (52151)	52151..52585	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
G6O57_RS24965 (52654)	52654..53755	-	1102	Protein_67	hypothetical protein	-
G6O57_RS24970 (53815)	53815..54612	-	798	WP_000544828.1	IS21-like element IS21 family helper ATPase IstB	-
G6O57_RS24975 (54612)	54612..55784	-	1173	WP_000952382.1	IS21-like element IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T150703 WP_001372321.1 NZ_CP049168:c51122-50997 [Shigella sonnei]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T150703 NZ_CP064923:c5629891-5629631 [Acaryochloris marina S15]
ATGGATACAGCTATTAGGACCCGCATCGTTAAAATTGGCAACTCCCAGGGTATTCGCATTCCCAAGCTTTTATTAGAGCA
AAGTGGTCTCAATGGAGAGGTTGAGATTACCGTGCAAGGAGACTGCTTAACCATTCGTACTGCTGAATGTTCTCGTGCTG
GGTGGGATGCAGCTTTTGCTTCCATGGCCCAACATCATGACGATGTGTTTTTAGATGAAGTCAACACTACCGACTGGGAT
GATACTGAATGGGAGTGGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT150703 NZ_CP049168:c51423-51199 [Shigella sonnei]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin