Detailed information of TA system TA150689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1669551..1670278
• Replicon: chromosome
• Accession: NZ_CP049167
• Organism: Shigella sonnei strain 09163633

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O57_RS08250
• Protein ID: WP_000547563.1
• Coordinates: 1669551..1669862 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O57_RS08255
• Protein ID: WP_000126296.1
• Coordinates: 1669859..1670278 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O57_RS08220 (1664705)	1664705..1666402	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
G6O57_RS08225 (1666412)	1666412..1666954	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O57_RS08230 (1666954)	1666954..1667721	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O57_RS08235 (1667718)	1667718..1668128	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O57_RS08240 (1668121)	1668121..1668591	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O57_RS08245 (1668616)	1668616..1669389	+	774	WP_001026459.1	hypothetical protein	-
G6O57_RS08250 (1669551)	1669551..1669862	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O57_RS08255 (1669859)	1669859..1670278	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O57_RS08260 (1670392)	1670392..1671816	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O57_RS08265 (1671825)	1671825..1673282	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O57_RS08270 (1673542)	1673542..1674552	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O57_RS08275 (1674700)	1674700..1675227	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150689 WP_000547563.1 NZ_CP049167:1669551-1669862 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150689 NZ_CP064923:655581-655835 [Acaryochloris marina S15]
ATGTGGACTGTTGTTCTTTCCAAGCAAGCTGCTAAAGATGCGAAAAAAGTCTCAGCATCTGATCTCAAGCCAAAAGTTCA
AAAACTGATAGCAGTATTACAGAGTGATCCTTATACTCTGCCCTACGAAAAGTTAGTGGGTGATATGCAGGGGTATTATT
CCCGAAGGATCAACATCCAGCACCGACTCGTTTACCAAATCTTTGATGAGCAAAAAGTGATCAAGATTTTGCGCATGTGG
AGCCGCTATGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150689 WP_000126296.1 NZ_CP049167:1669859-1670278 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150689 NZ_CP064923:655333-655581 [Acaryochloris marina S15]
ATGGATTTGTTGACCGTTCGAGAAGCCAGAGATAATTTATATCACCTGATTGATGAGATCGCTGAACATCACAAACCCAT
CGTGATCTCTGGTAAACGCAACGATGCGATCTTGATTTCTAAAGAAGATTGGGATGCTATCCAGGAAACAATTTATTTAA
GCAGTATTCCGGGGATGACTGACTCCATTCGGACGGCAGCCGCAGAACCTGTGGAAGAATGTATTGCCGTTGAAAATCTG
GATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22