Detailed information of TA system TA150681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4531018..4531745
• Replicon: chromosome
• Accession: NZ_CP049165
• Organism: Shigella sonnei strain 0401952027

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G6O56_RS22820
• Protein ID: WP_000547563.1
• Coordinates: 4531018..4531329 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6O56_RS22825
• Protein ID: WP_000126296.1
• Coordinates: 4531326..4531745 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O56_RS22790 (4526172)	4526172..4527869	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
G6O56_RS22795 (4527879)	4527879..4528421	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G6O56_RS22800 (4528421)	4528421..4529188	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
G6O56_RS22805 (4529185)	4529185..4529595	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G6O56_RS22810 (4529588)	4529588..4530058	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
G6O56_RS22815 (4530083)	4530083..4530856	+	774	WP_001026459.1	hypothetical protein	-
G6O56_RS22820 (4531018)	4531018..4531329	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6O56_RS22825 (4531326)	4531326..4531745	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G6O56_RS22830 (4531859)	4531859..4533283	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
G6O56_RS22835 (4533292)	4533292..4534749	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G6O56_RS22840 (4535009)	4535009..4536019	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
G6O56_RS22845 (4536167)	4536167..4536694	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T150681 WP_000547563.1 NZ_CP049165:4531018-4531329 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T150681 NZ_CP064908:827923-828279 [Synechococcus sp. CBW1107]
ATGAGGCTCTGGCTGGATGCCCAGCTGCCGCCAGCCCTGGCCCTCTGGATCGGTGCGCAGCCATGGGGAGTGGATGCCGT
CCCTGTGCGGGAGATCGGGTTGCGGGATGCCAGCGATCCGGAGATCTTTCGCCAGGCACGGGCCGCTGAGGCGGTGGTGA
TGACCAAAGACCGGGACTTCATCCGCCTGCTGGATGAGCAGAGCCCTCCTCCCCAGGTGATCTGGCTGCGGCTGGGGAAC
AGCAGCAATGCCGCACTGCAGCAGGTGTTGAGCACGACCCTGCTGCCAGCGCTGGAGCTGTTGCGCACCGGTGAACCCTG
GGTGGAGATCCGGCCGCAGGCCAACGCAGAGGCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT150681 WP_000126296.1 NZ_CP049165:4531326-4531745 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT150681 NZ_CP064908:827708-827926 [Synechococcus sp. CBW1107]
ATGGCGCCATCCCGTATCACCCACAACCCGGAGCAGTGCGGCGGTAAGCCCTGCATCCGCGGCATGCGCATTCGCGTGCG
GGATGTCCTGGAGCTCTATGCCGCTGGGCTGAGCAGCGAGCAGATCCTTGCCGACTACCCGGATCTGGAGCCGGAGGATC
TCAGTGCGGCACTGGATTACGCAGCCCGGGAGATCGACCATCCAGTGTTGGTGGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22