Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4254727..4255454 Replicon chromosome
Accession NZ_CP049165
Organism Shigella sonnei strain 0401952027

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G6O56_RS21460 Protein ID WP_000550189.1
Coordinates 4255140..4255454 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G6O56_RS21455 Protein ID WP_000560263.1
Coordinates 4254727..4255143 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O56_RS21445 (4250087) 4250087..4252438 + 2352 WP_000695471.1 alpha-glucosidase -
G6O56_RS21450 (4252664) 4252664..4254682 + 2019 WP_000121451.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G6O56_RS21455 (4254727) 4254727..4255143 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G6O56_RS21460 (4255140) 4255140..4255454 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G6O56_RS21465 (4255739) 4255739..4256875 - 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G6O56_RS21470 (4256960) 4256960..4257463 + 504 WP_001305117.1 M48 family metallopeptidase -
G6O56_RS21475 (4257540) 4257540..4258223 + 684 WP_001183060.1 vancomycin high temperature exclusion protein -
G6O56_RS21480 (4258302) 4258302..4259288 + 987 WP_001446103.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T150678 WP_000550189.1 NZ_CP049165:c4255454-4255140 [Shigella sonnei]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T150678 NZ_CP064879:c4509464-4509078 [Tolypothrix sp. PCC 7601]
ATGGTTGATCGGCCTTTGATTCAAGTTGACGCTTCGCTAACATTCAACCGAAATATACGCACTCTTGCTAAGAAATATCG
CAGTATTAGAAATGATATACAACCTGTTATCGAACAACTTCAGCAGGGAGAGTTACCAGGAGATCAAATACCTGGTGTTG
GTTATACAGTCTTCAAGTTGAGAGTCCGAAATAGCGACACTCAAAAAGGTAAAAGTGGTGGCTATCGTTTAATTTACTAC
GTCAAAACAGCAACAGGAATCATTTTACTGACCATTTACACAAAATCTGAACAAGTCGATATCGCCGCAGATGACATTCA
GAGCATCATTACAGACTATGAGCAACGGACTCTCACCGAGCAAGATGATATCTCAGACAATAGCTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT150678 WP_000560263.1 NZ_CP049165:c4255143-4254727 [Shigella sonnei]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT150678 NZ_CP064879:c4509699-4509457 [Tolypothrix sp. PCC 7601]
ATGAAAGCACTTAAAGTCATGGCAACGATTAACGATCAGGGACAACTAACTTTAGATCACCCGCTTTTAACTGATAAAAA
TAGCCGAGTAGAAGTCATTGTGCTAATCCCTGATGAAGAAGTTGTTCTGGATGATCAATCTCAAGCAGAAGTTTTAGCAG
ATTTTCGGCAAGCGTGGCACGAAGCCATGACTGGGCAAACTATCCCGGTGGCTCAAATGTGGGAAGGGCTAGAAGATGGT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References