Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2412238..2412779 Replicon chromosome
Accession NZ_CP049165
Organism Shigella sonnei strain 0401952027

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O56_RS12400 Protein ID WP_000615976.1
Coordinates 2412501..2412779 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O56_RS12395 Protein ID WP_000729704.1
Coordinates 2412238..2412498 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O56_RS12370 (2407707) 2407707..2409419 + 1713 Protein_2422 flagellar biosynthesis protein FlhA -
G6O56_RS12375 (2409542) 2409542..2410039 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O56_RS12380 (2410110) 2410110..2410238 - 129 Protein_2424 ISAs1 family transposase -
G6O56_RS12385 (2410293) 2410293..2410990 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
G6O56_RS12390 (2411279) 2411279..2412052 - 774 WP_127148796.1 C40 family peptidase -
G6O56_RS12395 (2412238) 2412238..2412498 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O56_RS12400 (2412501) 2412501..2412779 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O56_RS12405 (2412935) 2412935..2413675 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O56_RS12410 (2413646) 2413646..2414413 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6O56_RS12415 (2414619) 2414619..2415197 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150675 WP_000615976.1 NZ_CP049165:2412501-2412779 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150675 NZ_CP064875:2361344-2362084 [Bacillus toyonensis]
TTGACGATCTCTAACATTCGAATTGGCTTATGTATTTTAGCAATCGTTTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTATGAAGAGAAAAAGCAGCGAATTCGAAAAACTTGGTATGGATTATTCATAACATCAGTCACAGTTTATT
TCATGATTAAAGGAATCGATTTAACCCTCTGGAAAAATTTATTAATGTTCACTGCGATGGTCATTTTCGTTGATATTTCA
TTTATTTTAACACCTAACATTTCGGAAATATGGGGAGCGAAATTTAGCGATATCGGTAAAACTGTTCAATCCATAAAGCG
GTCATTAATCGCTTCAAAAGCAAGAGGGGAAATATATACGAACATTATCCAAAATATTAATCCAGCAGCATTTGGGACGA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAAATCGGAGCGAAA
ATTGTTGTATTTGAAGCAGCGAATGAATTAAATACGGGCTTTCGTGGTATTCGTTCTCAATTTAGTATTACAATACCTAT
AGAGCATATAGAGCAATTGAATGAACAAAAAGCAGTACAAGTAGAAAACGTGGGAATTATACCAGCAAAAATAATGAATG
ATGTTTTTATCGTTATTGATGGGAAGAAGAATAGCCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTATTTTAGTAAGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150675 WP_000729704.1 NZ_CP049165:2412238-2412498 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150675 NZ_CP064875:2362197-2362322 [Bacillus toyonensis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCTTTAATCAAAGGAGATAC
GTTGCAGCAAGTAGAAAAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References