Detailed information of TA system TA150643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2896245..2896892
• Replicon: chromosome
• Accession: NZ_CP049154
• Organism: Cronobacter sakazakii strain C105731

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G4U59_RS13630
• Protein ID: WP_069682249.1
• Coordinates: 2896545..2896892 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V5TYW5
• Locus tag: G4U59_RS13625
• Protein ID: WP_007776551.1
• Coordinates: 2896245..2896544 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4U59_RS13595 (G4U59_13530)	2891655..2892221	+	567	WP_063264316.1	DedA family protein	-
G4U59_RS13600 (G4U59_13535)	2892218..2892991	-	774	WP_004387792.1	SDR family oxidoreductase	-
G4U59_RS13605 (G4U59_13540)	2893164..2893247	+	84	WP_000691708.1	small membrane protein YohP	-
G4U59_RS13610 (G4U59_13545)	2893284..2894243	-	960	WP_105944196.1	MBL fold metallo-hydrolase	-
G4U59_RS13615 (G4U59_13550)	2894316..2895239	+	924	WP_054624201.1	LysR family transcriptional regulator	-
G4U59_RS13620 (G4U59_13555)	2895242..2896174	-	933	WP_007899538.1	tRNA dihydrouridine(16) synthase DusC	-
G4U59_RS13625 (G4U59_13560)	2896245..2896544	-	300	WP_007776551.1	XRE family transcriptional regulator	Antitoxin
G4U59_RS13630 (G4U59_13565)	2896545..2896892	-	348	WP_069682249.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4U59_RS13635 (G4U59_13570)	2897145..2897546	+	402	WP_007869367.1	CidA/LrgA family protein	-
G4U59_RS13640 (G4U59_13575)	2897543..2898238	+	696	WP_085660703.1	CidB/LrgB family autolysis modulator	-
G4U59_RS13645 (G4U59_13580)	2898368..2899252	+	885	WP_032988283.1	cytidine deaminase	-
G4U59_RS13650 (G4U59_13585)	2899377..2900090	+	714	WP_004387784.1	outer membrane permeability protein SanA	-
G4U59_RS13655 (G4U59_13590)	2900597..2901607	-	1011	WP_004387466.1	galactose/methyl galactoside ABC transporter permease MglC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13439.68 Da
• Isoelectric Point: 10.2057

>T150643 WP_069682249.1 NZ_CP049154:c2896892-2896545 [Cronobacter sakazakii]
MWTIYLTHEFECWLARQPQPLQEDVLAALGLLKIKGPHLGRPYADTLKGSRHAGMKELRVQHAGRPIRAFYAFDPHRYAI
VLCAAQKKGDEKRFYRVILRVADKLFSHYLNHREV

Nucleotide

• Length: 348 bp

>T150643 NZ_CP064855:470579-470899 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11035.67 Da
• Isoelectric Point: 5.1667

>AT150643 WP_007776551.1 NZ_CP049154:c2896544-2896245 [Cronobacter sakazakii]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLYALREALSVSQTEMARQMGIAQPSVAAIEQRGNEMKISTLKRYVEALG
GTVRLDVELPDGQRFGFTL

Nucleotide

• Length: 300 bp

>AT150643 NZ_CP064855:470219-470542 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S9UA72