Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2827936..2828581 Replicon chromosome
Accession NZ_CP049154
Organism Cronobacter sakazakii strain C105731

Toxin (Protein)


Gene name higB Uniprot ID A0A6L3QTA3
Locus tag G4U59_RS13320 Protein ID WP_004388208.1
Coordinates 2828219..2828581 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6L3TU83
Locus tag G4U59_RS13315 Protein ID WP_004388209.1
Coordinates 2827936..2828232 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4U59_RS13305 (G4U59_13240) 2825590..2826438 - 849 WP_097565810.1 DNA-3-methyladenine glycosylase 2 -
G4U59_RS13310 (G4U59_13245) 2826574..2827926 + 1353 WP_105944213.1 molecular chaperone -
G4U59_RS13315 (G4U59_13250) 2827936..2828232 - 297 WP_004388209.1 XRE family transcriptional regulator Antitoxin
G4U59_RS13320 (G4U59_13255) 2828219..2828581 - 363 WP_004388208.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G4U59_RS13325 (G4U59_13260) 2828812..2830059 + 1248 WP_105578723.1 MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit -
G4U59_RS13330 (G4U59_13265) 2830059..2833181 + 3123 WP_105578722.1 MdtB/MuxB family multidrug efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-86)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 14197.07 Da        Isoelectric Point: 8.0154

>T150642 WP_004388208.1 NZ_CP049154:c2828581-2828219 [Cronobacter sakazakii]
MAWTVIFTDRFNDWYQQQPEGLQDRIAALLWNLRYSGPLVGRPLVDTVKGSRYPNLKELRVQYGGEPWRVFFAFDAERQA
VVLCAGNKRSQKHFYDALIRQAEEEFFLHVQAMERRNENS

Download         Length: 363 bp

>T150642 NZ_CP064848:c125824-125519 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10837.75 Da        Isoelectric Point: 5.8787

>AT150642 WP_004388209.1 NZ_CP049154:c2828232-2827936 [Cronobacter sakazakii]
MKTLDDVMAGFSPERQAEILRMADEIALEHGLPRIREARAFSQQQLAEIMGVTQPAIAAIEQRGKEIKLLTLKRYVEALG
GKLSLLIELPEGSKVIPV

Download         Length: 297 bp

>AT150642 NZ_CP064848:c126044-125826 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6L3QTA3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L3TU83

References