Detailed information of TA system TA150633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2110653..2111218
• Replicon: chromosome
• Accession: NZ_CP049154
• Organism: Cronobacter sakazakii strain C105731

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3Q9CIP0
• Locus tag: G4U59_RS09805
• Protein ID: WP_012124820.1
• Coordinates: 2110653..2110931 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: G4U59_RS09810
• Protein ID: WP_004388462.1
• Coordinates: 2110931..2111218 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4U59_RS09790 (G4U59_09750)	2105716..2107653	+	1938	WP_105944605.1	methyl-accepting chemotaxis protein	-
G4U59_RS09795 (G4U59_09755)	2107762..2108748	+	987	WP_105504058.1	SDR family oxidoreductase	-
G4U59_RS09800 (G4U59_09760)	2108750..2110354	-	1605	WP_105944604.1	alpha-amylase family protein	-
G4U59_RS09805 (G4U59_09765)	2110653..2110931	+	279	WP_012124820.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4U59_RS09810 (G4U59_09770)	2110931..2111218	+	288	WP_004388462.1	HigA family addiction module antitoxin	Antitoxin
G4U59_RS09815 (G4U59_09775)	2111273..2113390	-	2118	WP_105944602.1	TonB-dependent receptor PqqU	-
G4U59_RS09820 (G4U59_09780)	2113668..2114726	+	1059	WP_105944601.1	YncE family protein	-
G4U59_RS09825 (G4U59_09785)	2114756..2115622	-	867	WP_105944600.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10628.13 Da
• Isoelectric Point: 10.4224

>T150633 WP_012124820.1 NZ_CP049154:2110653-2110931 [Cronobacter sakazakii]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGDRKGIWAVTVSGNWRITFEFRD
GDVFIVNYEDYH

Nucleotide

• Length: 279 bp

>T150633 NZ_CP064847:4271737-4272201 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTCCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10428.03 Da
• Isoelectric Point: 8.7791

>AT150633 WP_004388462.1 NZ_CP049154:2110931-2111218 [Cronobacter sakazakii]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFQA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT150633 NZ_CP064847:4271402-4271737 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3Q9CIP0