Detailed information of TA system TA150620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3430017..3430582
• Replicon: chromosome
• Accession: NZ_CP049144
• Organism: Cronobacter sakazakii strain C79

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3Q9CIP0
• Locus tag: G4U62_RS16115
• Protein ID: WP_012124820.1
• Coordinates: 3430017..3430295 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: G4U62_RS16120
• Protein ID: WP_004388462.1
• Coordinates: 3430295..3430582 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4U62_RS16100 (G4U62_16070)	3425082..3427019	+	1938	WP_148736318.1	methyl-accepting chemotaxis protein	-
G4U62_RS16105 (G4U62_16075)	3427127..3428113	+	987	WP_007888785.1	SDR family oxidoreductase	-
G4U62_RS16110 (G4U62_16080)	3428115..3429719	-	1605	WP_097641418.1	alpha-amylase family protein	-
G4U62_RS16115 (G4U62_16085)	3430017..3430295	+	279	WP_012124820.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4U62_RS16120 (G4U62_16090)	3430295..3430582	+	288	WP_004388462.1	HigA family addiction module antitoxin	Antitoxin
G4U62_RS16125 (G4U62_16095)	3430637..3432514	-	1878	Protein_3144	TonB-dependent receptor	-
G4U62_RS16130 (G4U62_16100)	3432703..3433881	+	1179	WP_216309921.1	site-specific integrase	-
G4U62_RS16135 (G4U62_16105)	3433862..3434053	-	192	WP_054628625.1	AlpA family transcriptional regulator	-
G4U62_RS16140 (G4U62_16110)	3434046..3434447	-	402	WP_084997683.1	hypothetical protein	-
G4U62_RS16145 (G4U62_16115)	3434444..3435310	-	867	WP_084997682.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10628.13 Da
• Isoelectric Point: 10.4224

>T150620 WP_012124820.1 NZ_CP049144:3430017-3430295 [Cronobacter sakazakii]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGDRKGIWAVTVSGNWRITFEFRD
GDVFIVNYEDYH

Nucleotide

• Length: 279 bp

>T150620 NZ_CP064847:903813-904211 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10428.03 Da
• Isoelectric Point: 8.7791

>AT150620 WP_004388462.1 NZ_CP049144:3430295-3430582 [Cronobacter sakazakii]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFQA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT150620 NZ_CP064847:903517-903810 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A3Q9CIP0