150611

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4439368..4440143 Replicon chromosome
Accession NZ_CP049140
Organism Pseudomonas nitroreducens strain HBP1

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7T1GPJ5
Locus tag G5B91_RS21195 Protein ID WP_011804662.1
Coordinates 4439368..4439826 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A6G6J080
Locus tag G5B91_RS21200 Protein ID WP_024766752.1
Coordinates 4439826..4440143 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5B91_RS21170 4434709..4435656 + 948 WP_023108457.1 TIGR03756 family integrating conjugative element protein -
G5B91_RS21175 4435666..4437060 + 1395 WP_009518521.1 integrating conjugative element protein -
G5B91_RS21180 4437057..4437416 + 360 WP_009518522.1 hypothetical protein -
G5B91_RS21185 4437431..4438948 + 1518 WP_011804664.1 conjugal transfer protein TraG N-terminal domain-containing protein -
G5B91_RS21190 4438964..4439341 - 378 WP_011804663.1 DUF3742 family protein -
G5B91_RS21195 4439368..4439826 - 459 WP_011804662.1 type II toxin-antitoxin system YhaV family toxin Toxin
G5B91_RS21200 4439826..4440143 - 318 WP_024766752.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
G5B91_RS21205 4440449..4442293 + 1845 WP_009876620.1 TraI domain-containing protein -
G5B91_RS21210 4442374..4445025 - 2652 WP_020924950.1 excinuclease ABC subunit UvrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4439368..4481819 42451


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-101)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17681.07 Da        Isoelectric Point: 10.3103

>T150611 WP_011804662.1 NZ_CP049140:c4439826-4439368 [Pseudomonas nitroreducens]
MQRHGWTLLFHDCVIEPLQKLQAAARRAQENDPKGFESNANVKLFRALSQLMLEVVPGDPARDEYRQGNTLGSAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T150611 NZ_CP064833:c725022-724819 [Morganella morganii subsp. morganii]
ATGACTAAACTGGACTATCTCATGCGCTTACGTAAGTGCACTTCTATTGAAACACTGGAACGTGTCATTGAGAAGAACAA
ATATGAACTGACTGATGATGAGCTGGAGGTTTTTTATTCAGCAGCCGATCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCATCTGTCTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11129.53 Da        Isoelectric Point: 4.2961

>AT150611 WP_024766752.1 NZ_CP049140:c4440143-4439826 [Pseudomonas nitroreducens]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKVAFDLRDGEVVVTRADAEHEDPAIGAFLSLLARDIEAGRNVQGLPED
LARAMLEHAGHGVNLDEEIDGEVEL

Download         Length: 318 bp

>AT150611 NZ_CP064833:c725445-725086 [Morganella morganii subsp. morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACCCTCATCAGAAGCAATAATTATTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGGCCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCGTTTAATGACGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAGGGGCATCTCGTCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1GPJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A6G6J080

References