Detailed information of TA system TA150573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3564871..3565565
• Replicon: chromosome
• Accession: NZ_CP049112
• Organism: Escherichia coli strain WF5-5-T2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: G6R06_RS17505
• Protein ID: WP_001263489.1
• Coordinates: 3564871..3565269 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G6R06_RS17510
• Protein ID: WP_000554758.1
• Coordinates: 3565272..3565565 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3560459)	3560459..3560539	-	81	NuclAT_12	-	-
- (3560459)	3560459..3560539	-	81	NuclAT_12	-	-
- (3560459)	3560459..3560539	-	81	NuclAT_12	-	-
- (3560459)	3560459..3560539	-	81	NuclAT_12	-	-
G6R06_RS17480 (3561135)	3561135..3561593	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G6R06_RS17485 (3561854)	3561854..3563311	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
G6R06_RS17490 (3563368)	3563368..3563889	-	522	Protein_3417	peptide chain release factor H	-
G6R06_RS17495 (3563885)	3563885..3564091	-	207	Protein_3418	RtcB family protein	-
G6R06_RS17500 (3564409)	3564409..3564861	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
G6R06_RS17505 (3564871)	3564871..3565269	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G6R06_RS17510 (3565272)	3565272..3565565	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G6R06_RS17515 (3565617)	3565617..3566672	-	1056	WP_001226164.1	DNA polymerase IV	-
G6R06_RS17520 (3566743)	3566743..3567528	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
G6R06_RS17525 (3567500)	3567500..3569212	+	1713	Protein_3424	flagellar biosynthesis protein FlhA	-
G6R06_RS17530 (3569436)	3569436..3569933	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T150573 WP_001263489.1 NZ_CP049112:c3565269-3564871 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T150573 NZ_CP064825:c494440-494222 [Escherichia coli UTI89]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT150573 WP_000554758.1 NZ_CP049112:c3565565-3565272 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT150573 NZ_CP064825:c494840-494466 [Escherichia coli UTI89]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3