Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 333983..334710 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G6R06_RS01560 Protein ID WP_000550189.1
Coordinates 334396..334710 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G6R06_RS01555 Protein ID WP_000560266.1
Coordinates 333983..334399 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS01545 (329243) 329243..331594 + 2352 WP_000695487.1 alpha-glucosidase -
G6R06_RS01550 (331920) 331920..333938 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G6R06_RS01555 (333983) 333983..334399 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G6R06_RS01560 (334396) 334396..334710 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G6R06_RS01565 (334994) 334994..336130 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G6R06_RS01570 (336215) 336215..336718 + 504 WP_001333820.1 M48 family metallopeptidase -
G6R06_RS01575 (336795) 336795..337487 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
G6R06_RS01580 (337566) 337566..338552 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T150552 WP_000550189.1 NZ_CP049112:c334710-334396 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T150552 NZ_CP064784:c3808791-3808438 [Klebsiella sp. BDA134-6]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGCTCAACGATACCGATAGGGCCAGCGTGCTTGC
AGCGCTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTCAGAGGCTCCCGCTACAGCA
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGTATT
GTGCTTTGCGCCGGAAATAAGGTCAGCAATGAAAAACGTTTTTACGATGAGATTCTCCCGGTGGCTGACCGGGAATATAC
CAACTGGCTGAACACATTAAATGAGAAGGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT150552 WP_000560266.1 NZ_CP049112:c334399-333983 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT150552 NZ_CP064784:c3808433-3808131 [Klebsiella sp. BDA134-6]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTTGTGGCCGAAGCCGAGGCTATGGCTAAGGATAT
TTTGCTCAATATTCATCTTGCTGAGTTGCGTGAGAGGGTGCAGAAAACGCAGGTGGAAATGGCGCAGGAATTAGGTATCA
AGCAGCCTACCGTCGCGGGAATGGAAAAACCGGGCCGCGACCTGAAGCTCTCCACGCTCAAACGTTACGTTGAAGCGGCG
GGCGGTAAGCTAAGCCTCAATGTTGAGCTGCCGGATGGGTCGCATTACGGGTTTGTACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References