Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 71427..72084 | Replicon | plasmid pMCR_NDM |
| Accession | NZ_CP049111 | ||
| Organism | Escherichia coli strain WF5-5-T1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | G5631_RS22740 | Protein ID | WP_000270043.1 |
| Coordinates | 71734..72084 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | G5631_RS22735 | Protein ID | WP_000124640.1 |
| Coordinates | 71427..71729 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G5631_RS22685 (G5631_22470) | 67052..67480 | + | 429 | WP_072713905.1 | hypothetical protein | - |
| G5631_RS22690 (G5631_22475) | 67537..67896 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| G5631_RS22695 (G5631_22480) | 67896..68342 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| G5631_RS22700 (G5631_22485) | 68339..68857 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| G5631_RS22705 (G5631_22490) | 68857..69087 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| G5631_RS22710 (G5631_22495) | 69074..69931 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| G5631_RS22715 (G5631_22500) | 69957..70145 | + | 189 | WP_001270411.1 | hypothetical protein | - |
| G5631_RS22720 (G5631_22505) | 70162..70689 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| G5631_RS22725 (G5631_22510) | 70747..71019 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| G5631_RS22730 (G5631_22515) | 71107..71400 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| G5631_RS22735 (G5631_22520) | 71427..71729 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| G5631_RS22740 (G5631_22525) | 71734..72084 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| G5631_RS22745 (G5631_22530) | 72247..72795 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| G5631_RS22750 (G5631_22540) | 73136..73330 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| G5631_RS22755 (G5631_22545) | 73341..73712 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| G5631_RS22760 (G5631_22550) | 73705..74175 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| G5631_RS22765 (G5631_22555) | 74190..74525 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| G5631_RS22770 (G5631_22560) | 74622..75110 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| G5631_RS22775 (G5631_22565) | 75113..75610 | + | 498 | WP_000062185.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaNDM-4 / sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / fosA3 / blaCTX-M-14 / mcr-1.1 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / sul2 / aph(4)-Ia / aac(3)-IVa / mph(A) / tet(M) | - | 1..436531 | 436531 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T150547 WP_000270043.1 NZ_CP049111:c72084-71734 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T150547 NZ_CP064781:c2414581-2414255 [Azospira restricta]
ATGTCTTTCAACATCGAATACTTCCACGAACGAGTCCTCCATGAGGTCGAGTCGTGGCCGGTCGATGTTCTGGCTGACTA
CGCGCGGCTCGTCGAGTTGCTTGCGGAGCATGGCCCGAGCCTTCGGCTTCCGCATTCGCGCGCCTTTGGTGATGGCCTGT
TTGAATTGCGCCCCAAGGGCCGTAGCGGTATTGGCCGTGCGTTTTACTGTTTTTTGCTCGGCAAGCGCGTCATCGTGCTG
CATGCGTTCATCAAGAAAACGCAGCAGACACCGGACCGCGAGTTGAAGCTGGCGCGTCAGCGCCTGAAGGAGGTTCGAGA
TGCCTGA
ATGTCTTTCAACATCGAATACTTCCACGAACGAGTCCTCCATGAGGTCGAGTCGTGGCCGGTCGATGTTCTGGCTGACTA
CGCGCGGCTCGTCGAGTTGCTTGCGGAGCATGGCCCGAGCCTTCGGCTTCCGCATTCGCGCGCCTTTGGTGATGGCCTGT
TTGAATTGCGCCCCAAGGGCCGTAGCGGTATTGGCCGTGCGTTTTACTGTTTTTTGCTCGGCAAGCGCGTCATCGTGCTG
CATGCGTTCATCAAGAAAACGCAGCAGACACCGGACCGCGAGTTGAAGCTGGCGCGTCAGCGCCTGAAGGAGGTTCGAGA
TGCCTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT150547 WP_000124640.1 NZ_CP049111:c71729-71427 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT150547 NZ_CP064781:c2414262-2413945 [Azospira restricta]
ATGCCTGATCTGAAATTCAACCCTGTGGCCCATGACCACAAGAAGTTTCTTGCCAAGGCGCAGAAGCGCGACGGCTTTAC
CGAAGCCTACGAGGCGCTTGCGCTCGAATATCAGCTTGCCAGCCAGATGCTGAAGGCGAGAGCGCGAGCCGGTCTTACGC
AGGATGCGGTTGCCGAACGCATGGGTACGACAAAAAGTGCTATTTCCCGACTCGAGTCCGCCGGCAAGCATGCGCCATCG
CTCGCCACGCTAAAGCGTTACGCCGAAGCGGTCGGCTGCGATCTTCAAGTCAAGTTGGTACCACACAAAGCCGCATGA
ATGCCTGATCTGAAATTCAACCCTGTGGCCCATGACCACAAGAAGTTTCTTGCCAAGGCGCAGAAGCGCGACGGCTTTAC
CGAAGCCTACGAGGCGCTTGCGCTCGAATATCAGCTTGCCAGCCAGATGCTGAAGGCGAGAGCGCGAGCCGGTCTTACGC
AGGATGCGGTTGCCGAACGCATGGGTACGACAAAAAGTGCTATTTCCCGACTCGAGTCCGCCGGCAAGCATGCGCCATCG
CTCGCCACGCTAAAGCGTTACGCCGAAGCGGTCGGCTGCGATCTTCAAGTCAAGTTGGTACCACACAAAGCCGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|