Detailed information of TA system TA150542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3878187..3878599
• Replicon: chromosome
• Accession: NZ_CP049110
• Organism: Escherichia coli strain WF5-5-T1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: G5631_RS18980
• Protein ID: WP_000132601.1
• Coordinates: 3878258..3878599 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3878187..3878263 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5631_RS18970 (3875050)	3875050..3876639	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
G5631_RS18975 (3876636)	3876636..3878030	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (3878187)	3878187..3878263	-	77	NuclAT_14	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_14	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_14	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_14	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_15	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_15	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_15	-	Antitoxin
- (3878187)	3878187..3878263	-	77	NuclAT_15	-	Antitoxin
G5631_RS18980 (3878258)	3878258..3878599	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
G5631_RS18985 (3878761)	3878761..3880140	+	1380	Protein_3706	5-methylcytosine-specific restriction endonuclease subunit McrB	-
G5631_RS18990 (3880140)	3880140..3881186	+	1047	WP_278147151.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T150542 WP_000132601.1 NZ_CP049110:3878258-3878599 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T150542 NZ_CP064780:c3412973-3412050 [Xanthomonas oryzae pv. oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACCGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT150542 NZ_CP049110:c3878263-3878187 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin