Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3569947..3570488 Replicon chromosome
Accession NZ_CP049110
Organism Escherichia coli strain WF5-5-T1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag G5631_RS17535 Protein ID WP_000615983.1
Coordinates 3570210..3570488 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag G5631_RS17530 Protein ID WP_000729703.1
Coordinates 3569947..3570207 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5631_RS17510 (3565622) 3565622..3566407 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
G5631_RS17515 (3566379) 3566379..3568091 + 1713 Protein_3422 flagellar biosynthesis protein FlhA -
G5631_RS17520 (3568315) 3568315..3568812 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
G5631_RS17525 (3568988) 3568988..3569737 - 750 WP_000056849.1 C40 family peptidase -
G5631_RS17530 (3569947) 3569947..3570207 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G5631_RS17535 (3570210) 3570210..3570488 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G5631_RS17540 (3570644) 3570644..3571384 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G5631_RS17545 (3571355) 3571355..3572122 - 768 WP_000333380.1 class II glutamine amidotransferase -
G5631_RS17550 (3572328) 3572328..3572906 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T150541 WP_000615983.1 NZ_CP049110:3570210-3570488 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150541 NZ_CP064780:c2923746-2923495 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT150541 WP_000729703.1 NZ_CP049110:3569947-3570207 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150541 NZ_CP064780:c2924051-2923773 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References