Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3563750..3564444 | Replicon | chromosome |
| Accession | NZ_CP049110 | ||
| Organism | Escherichia coli strain WF5-5-T1 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | G5631_RS17495 | Protein ID | WP_001263489.1 |
| Coordinates | 3563750..3564148 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | G5631_RS17500 | Protein ID | WP_000554758.1 |
| Coordinates | 3564151..3564444 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3559338) | 3559338..3559418 | - | 81 | NuclAT_12 | - | - |
| - (3559338) | 3559338..3559418 | - | 81 | NuclAT_12 | - | - |
| - (3559338) | 3559338..3559418 | - | 81 | NuclAT_12 | - | - |
| - (3559338) | 3559338..3559418 | - | 81 | NuclAT_12 | - | - |
| G5631_RS17470 (3560014) | 3560014..3560472 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| G5631_RS17475 (3560733) | 3560733..3562190 | + | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| G5631_RS17480 (3562247) | 3562247..3562768 | - | 522 | Protein_3415 | peptide chain release factor H | - |
| G5631_RS17485 (3562764) | 3562764..3562970 | - | 207 | Protein_3416 | RtcB family protein | - |
| G5631_RS17490 (3563288) | 3563288..3563740 | - | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| G5631_RS17495 (3563750) | 3563750..3564148 | - | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| G5631_RS17500 (3564151) | 3564151..3564444 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| G5631_RS17505 (3564496) | 3564496..3565551 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| G5631_RS17510 (3565622) | 3565622..3566407 | - | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| G5631_RS17515 (3566379) | 3566379..3568091 | + | 1713 | Protein_3422 | flagellar biosynthesis protein FlhA | - |
| G5631_RS17520 (3568315) | 3568315..3568812 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC | 3492285..3569614 | 77329 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T150540 WP_001263489.1 NZ_CP049110:c3564148-3563750 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T150540 NZ_CP064780:c1128140-1127811 [Xanthomonas oryzae pv. oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT150540 WP_000554758.1 NZ_CP049110:c3564444-3564151 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT150540 NZ_CP064780:c1127814-1127422 [Xanthomonas oryzae pv. oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCTGAGGTTTCCCCACATTTC
TTCCTGCGAGATCAAGCACTTGTTGCATCAAGGCGTGGAAGAAGGTGCGCGCATGGGGCACGCTTCAAGGTGA
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCTGAGGTTTCCCCACATTTC
TTCCTGCGAGATCAAGCACTTGTTGCATCAAGGCGTGGAAGAAGGTGCGCGCATGGGGCACGCTTCAAGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A090J8B1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |