Detailed information of TA system TA150504

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120322..120743
• Replicon: plasmid p2
• Accession: NZ_CP049102
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: G5V60_RS24940
• Protein ID: WP_001302184.1
• Coordinates: 120322..120480 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	120545..120743 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS24900	115387..115602	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
G5V60_RS24905	115738..116385	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
G5V60_RS24910	116577..116960	-	384	WP_001063020.1	relaxosome protein TraM	-
G5V60_RS24915	117312..117902	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
G5V60_RS24920	118197..119018	-	822	WP_001234475.1	DUF945 domain-containing protein	-
G5V60_RS24925	119137..119424	-	288	WP_000107546.1	hypothetical protein	-
G5V60_RS25760	119449..119655	-	207	WP_000547965.1	hypothetical protein	-
G5V60_RS24935	119725..120084	+	360	Protein_119	hypothetical protein	-
G5V60_RS24940	120322..120480	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	120545..120743	-	199	NuclAT_0	-	Antitoxin
-	120545..120743	-	199	NuclAT_0	-	Antitoxin
-	120545..120743	-	199	NuclAT_0	-	Antitoxin
-	120545..120743	-	199	NuclAT_0	-	Antitoxin
G5V60_RS24945	120755..121474	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
G5V60_RS24950	121471..121905	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
G5V60_RS24955	121960..123918	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
G5V60_RS24960	123984..124217	-	234	WP_000005990.1	DUF905 family protein	-
G5V60_RS24965	124274..124813	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
G5V60_RS24970	125131..125640	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..168051	168051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T150504 WP_001302184.1 NZ_CP049102:c120480-120322 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T150504 NZ_CP064760:c517036-516638 [Microbacterium schleiferi]
ATGACGATCGCCCTCGACACGTCAGCGTTGATGGCCATCGTCCTTGGCGAAGCGGATGCGTCGCGTTTCGCCCGCGTGAT
CGCCGAGAACGTTGGCGACCTTCACGTGAGCGCTGCGACGCTCGTGGAAGCGACGATCGTGGCCGATGCGCGTCAGGGAC
CGGCGGCTACGCAGGATCTCGAGGCCCTCATCTCTCGCGCTCGCGTCACGGTCGCGCCCTTCGACGCCGCTCAAGCGTCG
ATCGCAACCGCAGCGTGGCGCCGATTCGGTAAGGGTCGTCACGCAGCATCACTGAACATGGGTGACTGCTACTCGTATGC
GCTCGCCCGCAGCCTGGGCGCTGCACTGCTCTTCAAGGGCGACGACTTCGCGCAGACCGACATCGCCGCTGCGATCTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT150504 NZ_CP049102:c120743-120545 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin