Detailed information of TA system TA150501

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82168..82422
• Replicon: plasmid p2
• Accession: NZ_CP049102
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: G5V60_RS24715
• Protein ID: WP_001351576.1
• Coordinates: 82168..82374 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82361..82422 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS24690	77450..80455	+	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
G5V60_RS24695	80388..80853	-	466	Protein_70	incFII family plasmid replication initiator RepA	-
G5V60_RS24700	80846..81328	-	483	WP_001273588.1	hypothetical protein	-
G5V60_RS24705	81321..81395	-	75	WP_001442103.1	RepA leader peptide Tap	-
G5V60_RS24710	81627..81884	-	258	WP_000083845.1	replication regulatory protein RepA	-
G5V60_RS24715	82168..82374	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	82361..82422	+	62	NuclAT_1	-	Antitoxin
-	82361..82422	+	62	NuclAT_1	-	Antitoxin
-	82361..82422	+	62	NuclAT_1	-	Antitoxin
-	82361..82422	+	62	NuclAT_1	-	Antitoxin
G5V60_RS25755	82678..82752	-	75	Protein_75	endonuclease	-
G5V60_RS24720	82998..83210	-	213	WP_001299730.1	ANR family transcriptional regulator	-
G5V60_RS24725	83346..83906	-	561	WP_000139315.1	fertility inhibition protein FinO	-
G5V60_RS24730	84009..84869	-	861	WP_000704513.1	alpha/beta hydrolase	-
G5V60_RS24735	84928..85674	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..168051	168051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T150501 WP_001351576.1 NZ_CP049102:c82374-82168 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T150501 NZ_CP064707:c4024898-4024503 [Bradyrhizobium sp. 131]
ATGGTGCTGGATACGTCTGCGATCGTCGCGACCGTCACCAACGAGCCAGATGGGCAGCGCTATCGGAAAGCCATGCTGGA
TGCTGCCAGCCTGTCGATGTCAGCAGTAGCAATCCTGGAAACCAAGATCGTTCTCTCTGCTCGCTTCGGAGCATCGGCCG
TGAAGTTCTTCGACGAATTGCTGGAGATTGCCGGGATTGTCGTAGTGCCGTTCGACGCAGACATGGCGAAAGGCGCGTTC
GAGGCATTTCAACGCTTCGGCAAGGGTCGGGGGACATCCGGCGCAGCTCAACATCGTCGATTGTGCGGTCTACGCTTGGC
GAAAGCGCGTTCGCAACCGCTGCTCTTCAAGGGAGCCCACTTTGCTAAGACAGACATTCTCCCCGCGTTGACCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT150501 NZ_CP049102:82361-82422 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin