Detailed information of TA system TA150499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4794380..4794982
• Replicon: chromosome
• Accession: NZ_CP049101
• Organism: Escherichia coli strain EC28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: G5V60_RS23295
• Protein ID: WP_000897303.1
• Coordinates: 4794671..4794982 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5V60_RS23290
• Protein ID: WP_000356397.1
• Coordinates: 4794380..4794670 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5V60_RS23260	4789685..4790614	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
G5V60_RS23265	4790796..4791038	-	243	WP_001068515.1	ribbon-helix-helix domain-containing protein	-
G5V60_RS23270	4791328..4792176	+	849	WP_001528046.1	hypothetical protein	-
G5V60_RS23275	4792492..4792941	+	450	Protein_4543	hypothetical protein	-
G5V60_RS23280	4793126..4793344	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
G5V60_RS23285	4793743..4794021	-	279	WP_001296612.1	hypothetical protein	-
G5V60_RS23290	4794380..4794670	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G5V60_RS23295	4794671..4794982	-	312	WP_000897303.1	hypothetical protein	Toxin
G5V60_RS23300	4795211..4796119	+	909	WP_001385591.1	alpha/beta hydrolase	-
G5V60_RS23305	4796183..4797124	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G5V60_RS23310	4797169..4797606	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G5V60_RS23315	4797603..4798475	-	873	WP_000920763.1	virulence factor BrkB family protein	-
G5V60_RS23320	4798469..4799068	-	600	WP_001298594.1	glucose-1-phosphatase	-
G5V60_RS23325	4799167..4799952	-	786	WP_001523571.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T150499 WP_000897303.1 NZ_CP049101:c4794982-4794671 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T150499 NZ_CP064706:33453-33722 [Bradyrhizobium sp. 155]
GTGCAGGACGGTGAGGAGCCAATAGCCGCAAAGGCGCTGAGAGGATTCGGCGGGCGTGCTATCCTGGAGCTAGTCGACGA
TTTCGACAGCAAGACCTACCGGGCCGTTTATACGGTGCGCTTTGCCGGAATGGTGTACGTGCTCCCCGTTTTTCAGAAGA
AGGCGAAGAAGGGCATCGCCACTTCCCAGCAAGACATTGAACCTATCAAAACGCGTCTGCGCGATGCTGAAGAGCATTAT
CGTGCGCTGGCAGACAAAGGAGGCCGCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT150499 WP_000356397.1 NZ_CP049101:c4794670-4794380 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT150499 NZ_CP064706:33725-34063 [Bradyrhizobium sp. 155]
ATGACCAAACGCAAAATCGAAGTTTACGAGGGCAGCGGCAATATATTCGCCGATCTTGGGTTGCCCAATCCAGAAGAACG
GCTGCTGAAATCCAGCATCGTGACCGAGCTGCATCGCCTGATCAAGGCACGCAACCTGACCCAGATCAAAGCGGCAAAGC
TGATCGGGATTTCGCAGCCTGACCTGTCTCACCTGCTCCGTGGTGACTACCATAACTATTCAGCCGAGCGCCTGATGAAA
ATGCTCACGGTGTTCGAGCAGCGTATCGAAATCGTGATGCGGCCTCACCGCAAGGCCGGTGAGCCGGGCCGTATCACCTT
CAAACCTGTTGCAGCGTGA

Structures

Toxin

• AlphaFold DB: A0A3L4YH82