Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3617564..3618243 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag G5V60_RS17835 Protein ID WP_000057523.1
Coordinates 3617941..3618243 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag G5V60_RS17830 Protein ID WP_000806442.1
Coordinates 3617564..3617905 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS17820 3613808..3614740 - 933 WP_001524187.1 glutaminase A -
G5V60_RS17825 3615002..3617506 + 2505 WP_001524186.1 copper-exporting P-type ATPase CopA -
G5V60_RS17830 3617564..3617905 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
G5V60_RS17835 3617941..3618243 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G5V60_RS17840 3618376..3619170 + 795 WP_000365147.1 TraB/GumN family protein -
G5V60_RS17845 3619374..3619853 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
G5V60_RS25740 3619890..3621547 - 1658 Protein_3486 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
G5V60_RS17860 3621765..3622985 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3598344..3618243 19899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T150495 WP_000057523.1 NZ_CP049101:c3618243-3617941 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T150495 NZ_CP064703:7765470-7765748 [Bradyrhizobium sp. 170]
ATGGCACACGTTTCTTACACTGAACTGCGTAACAACCTCGCGTCCTACATGGACGAGGTTTGCGATGATCGCGCCCCCCT
GTTCGTCACCCGCCAGAATGCCCGCACCGTCGTTCTCATGGCCGAGGATGAGTATGAGAGTCTCATGGAGACCGTCCACC
TCCTGAAGAGTCCCGCCAATGCAGTACGTCTTCTACGTTCCATCAAGGAAGCGGACGAGGGCAAACTAACTGAACGCGAG
TTGATCGAGCCAACAAAGGCAGGTTCGGCCAAGGTATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT150495 WP_000806442.1 NZ_CP049101:c3617905-3617564 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT150495 NZ_CP064703:7765745-7766011 [Bradyrhizobium sp. 170]
ATGAAAACCGCTTGGACGGAAGATGCCTGGCAAGACTATCTGCAATGGCAAAAGGAGGATGAAAAGATCCTCGAAGTCAT
AAACGGTCTAATCAAAGATATTAAGCGCGATCCGTTCAAGGGGCTTGGGAAACCAGAGCCCCTTAAACATGCGCTCCAGG
GATGGTGGTCGCGCCGCATTTCAGGCGAACATCGCTTGGTGTATCGCGTTTCTGGAAAGGGTGACACTCAGCAACTCGAC
ATTGCTCAGTGCCGCTATCATTACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References