Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2554426..2554988 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag G5V60_RS12405 Protein ID WP_000605675.1
Coordinates 2554426..2554704 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag G5V60_RS12410 Protein ID WP_000781370.1
Coordinates 2554704..2554988 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS12380 2550014..2550445 - 432 WP_000152305.1 peroxiredoxin OsmC -
G5V60_RS12385 2550789..2551004 + 216 WP_000495774.1 biofilm-dependent modulation protein -
G5V60_RS12390 2551106..2551243 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
G5V60_RS12395 2551399..2553096 + 1698 WP_000433462.1 malate dehydrogenase -
G5V60_RS12400 2553230..2554240 + 1011 WP_000642417.1 alcohol dehydrogenase AdhP -
G5V60_RS12405 2554426..2554704 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G5V60_RS12410 2554704..2554988 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
G5V60_RS12415 2555216..2555869 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
G5V60_RS12420 2555862..2556746 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
G5V60_RS12425 2556759..2559806 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T150494 WP_000605675.1 NZ_CP049101:2554426-2554704 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T150494 NZ_CP064703:c5979326-5978433 [Bradyrhizobium sp. 170]
ATGCCTAAGAAGCCGAAACCACCGATCAAGAATATGGGCGCGTCAGTTCGGGCGCGACTGCTCAACCTTTCAAAACAACG
CCGCCAACCCTTCGATCTGCTGCTCAACAACTACGTTCTAGAGAGGCTTCTCTATCGCTTGAGCCAGACCCAGTATCGCG
ATCGCTTCATTCTGAAAGGGGCGATGCTCCTTACAAAGTGGTTTGAAGATCCGCTACGTCCCACTCGCGACCTCGACCTC
CTCGGCATCGGTAACGACGATCAAGATGAGATGGTCCGCATCTTTCAGGAGGTCTGTGGCGCCTCCTACAACGACGGCGT
CGTCTTTGATTCAAACAGCGTGAAAGTCGATCGCATTCGCGAAGAAACGGAATATGGCGGTTTGCGCATTACAGCGACGG
CCAAGGTTGATACGGCCCAAGTACGCGTCGTTATTGATATCGCCTTTGGCGATTCAGTCGAACCCGGCCTTCAGGAAATA
GACCTGCCCGTTCTGTTGGATTTTCCTGCGCCAAACTTGCGCGCCTACGCTCGCGAGACTGTTATCGCCGAAAAATTCCA
AGCAATGGTGATGTTGGGTCGCGCCAACAGCCGAATGAAGGATTTGTACGATATTTGGGTGCTATCGCGTAATTTCGAAT
TCAAGGGCGACACGCTGCCGCGAGCCATCGCTGCGACCTTCGCCCGGCGCAAGACCGAAATTCCCAAAGAGATTCCGGAC
GCCTTGACGACAGCTTTCGCGGAAGACCCTACGAAAGTCGCGCAATGGAATTCGTTCATCGAAGACGTCGCGTTTCAGCC
GGGGATTTTGGCCGATGTCATCAACGATCTAAATAAGTTTCTCATGCCCCAAGCGTCAGCGGCACACGCGATCAATAAGA
AAAACTCGACGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT150494 WP_000781370.1 NZ_CP049101:2554704-2554988 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT150494 NZ_CP064703:c5979963-5979319 [Bradyrhizobium sp. 170]
ATGCCTTCGCTTTCCAGAACTCAGCAAGAGCGCGCTCTCTCCCTGCTCAAGCAGCAGGGCATGGTCCGCCTTTCCGAATT
TGTAGAAGCTGGCATCACCGCAACCACCGTTGCGCGGATGAAGGACAAAGGGCTTGTCCTCCAGCTCAGTCGAGGACTGT
ACCAACTTCCCGACGCCCCGATCGACACACATCATACGCTCGCCGAGGCAGCCAAACTTGTCCCGAAGGGGGTCGTTTGT
CTTACGTCCGCACTGGCCTTTCACGGCCTGACCGACGCGATCCCATCGCGCGTTTGGATAGCCATTGGCGCAAAGGACCG
GAGGCCTCGCATCGAAACACCGCCCCTTCAATTCGTGCGTTTCGGCGAAAAGGTTCTAACCTCCGGCATCAAAGAGCACG
TCATCGAAGGCGTGCGCGTTCGTATTTATATTCCCGCAAAGACGGTGGTCGATCTATTTCGCTATCGGCGCCCCGCCGGC
AACCGCTATCAAAAGAGCCCAGGTCTCAATCTCGCACTCGAAGGTCTGCGAGAAGCCCTGCGCCGGCGCAAGGCATCTCC
CGCCGAAATCGCCCGCTACGCCAATGAAGCCGGAGTCTGGAAGGTCGTGCAGCCCTATCTGGAGGCTATGACTGCCAATG
CCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References