Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1965340..1965842 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag G5V60_RS09335 Protein ID WP_164699364.1
Coordinates 1965588..1965842 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G5V60_RS09330 Protein ID WP_001259255.1
Coordinates 1965340..1965591 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS09305 1960518..1961585 - 1068 WP_000080115.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G5V60_RS09310 1961585..1962655 - 1071 WP_164699362.1 histidinol-phosphate transaminase -
G5V60_RS09315 1962652..1963956 - 1305 WP_164699363.1 histidinol dehydrogenase -
G5V60_RS09320 1963962..1964861 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
G5V60_RS09325 1965007..1965057 - 51 WP_001364200.1 his operon leader peptide -
G5V60_RS09330 1965340..1965591 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G5V60_RS09335 1965588..1965842 + 255 WP_164699364.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G5V60_RS09340 1965925..1966749 + 825 WP_000754740.1 SDR family oxidoreductase -
G5V60_RS09345 1966795..1967724 + 930 WP_045133079.1 LysR family transcriptional regulator -
G5V60_RS09350 1967939..1968001 + 63 WP_010723108.1 membrane protein YoeI -
G5V60_RS09355 1967991..1969349 + 1359 WP_164699365.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10225.66 Da        Isoelectric Point: 8.0353

>T150483 WP_164699364.1 NZ_CP049101:1965588-1965842 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFESKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDLLLIAAC
RYHY

Download         Length: 255 bp

>T150483 NZ_CP064692:c2971846-2971571 [Bradyrhizobium sp. 191]
GTGCAGGAAGGTGAGGAGCCAATAGCCGCAAAGGCGCTGAGAGGATTCGGCGGGCGTGCTATCCAGGAGCTAGTCGATGA
TTTCGACAGCAAGACCTGCCGGGCCGTTTATACGGTGCGCTTTGCCGGAATGGTGTACGTGCTCCACGCTTTTCAGAAGA
AGGCGAAGAAGGGCATCGCCACTTCCCAGCAAGACATTGAACCTATCAAATCGCGCCTGCGCGATGCTGAAGAGCATTAT
CGTGCGCGGGCAGACAAAGGAGGCCGCAAGCCATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT150483 WP_001259255.1 NZ_CP049101:1965340-1965591 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT150483 NZ_CP064692:c2971574-2971236 [Bradyrhizobium sp. 191]
ATGACCAAACGCAAAATCGAAGCCTACGAGGGCAGCGGCAATATATTGGCCGATCTTGGGTTGCCCAATCCAGAAGAACG
GCTGCTGAAATCCAGCATCGTGACCGAGCTGCATCGCCTGATCAAGGCACGCAACCTGACCCAGGTCAAGGCGGCAAAGC
TGATCGGGATTTCGCAGCCTGACCTGTCTCACCTGCTGCGTGGTGACTACAGTGACTATTCAGCCGAGCGCCTGATGAAA
ATGCTCACGGTGTTCGAGCAGGATATCGAGATCGTGATGCGGCCTCACCGCAAGGCCGGTGAGCCGGGCCGTATCACCTT
CAAACCTGTTGCAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References