Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 620875..621674 Replicon chromosome
Accession NZ_CP049101
Organism Escherichia coli strain EC28

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag G5V60_RS03040 Protein ID WP_000347251.1
Coordinates 620875..621339 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag G5V60_RS03045 Protein ID WP_001296435.1
Coordinates 621339..621674 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5V60_RS03010 615876..616310 - 435 WP_000948837.1 PTS sugar transporter subunit IIA -
G5V60_RS03015 616328..617206 - 879 WP_001525735.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G5V60_RS03020 617196..617975 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G5V60_RS03025 617986..618459 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G5V60_RS03030 618482..619762 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
G5V60_RS03035 620011..620820 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
G5V60_RS03040 620875..621339 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G5V60_RS03045 621339..621674 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G5V60_RS03050 621823..623394 - 1572 WP_001525733.1 galactarate dehydratase -
G5V60_RS03055 623769..625103 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
G5V60_RS03060 625119..625889 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T150480 WP_000347251.1 NZ_CP049101:c621339-620875 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T150480 NZ_CP049101:c621339-620875 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT150480 WP_001296435.1 NZ_CP049101:c621674-621339 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT150480 NZ_CP049101:c621674-621339 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References