Detailed information of TA system TA150468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1221306..1221949
• Replicon: chromosome
• Accession: NZ_CP049090
• Organism: Glaesserella parasuis strain aHPS7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: G5S31_RS05895
• Protein ID: WP_005714197.1
• Coordinates: 1221605..1221949 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5S31_RS05890
• Protein ID: WP_012621770.1
• Coordinates: 1221306..1221602 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S31_RS05865	1216900..1217322	-	423	WP_043894462.1	helix-turn-helix transcriptional regulator	-
G5S31_RS05870	1217469..1218461	+	993	WP_164689524.1	DUF3944 domain-containing protein	-
G5S31_RS05875	1218492..1219700	+	1209	WP_082259179.1	IS256 family transposase	-
G5S31_RS05880	1219707..1219925	+	219	WP_164689526.1	hypothetical protein	-
G5S31_RS05885	1219885..1221093	-	1209	WP_082259179.1	IS256 family transposase	-
G5S31_RS05890	1221306..1221602	-	297	WP_012621770.1	helix-turn-helix domain-containing protein	Antitoxin
G5S31_RS05895	1221605..1221949	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G5S31_RS05900	1222164..1224143	+	1980	WP_164689528.1	exoribonuclease II	-
G5S31_RS05905	1224253..1225344	+	1092	WP_164689530.1	murein transglycosylase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T150468 WP_005714197.1 NZ_CP049090:c1221949-1221605 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T150468 NZ_CP064686:c711842-711546 [Bradyrhizobium sp. 4]
ATACCGACAATCAAGAGAACCGACGAATTCTCTGACTGGCTTAGAAAACAGCGAGACCGGAGAGCGAAGGCAAAAATCCT
CGCCCGGATCGATCGGCTGGCGCTCGGCAATCCCGGAGACGTTGCTGCGGTCGGAGGCGGCATCAGCGAAATGAGAGTCC
ATGAGGGCGCCGGATACCGGATCTACTACGTTGCCCGAGGCGAGGAGATCATCGTCCTTCTTTGCGGTGGCGATAAAGGC
TCTCAGCCGGGGGACATAGCGTTGGCAAAGAAGATCGCGAGCGAATTGGAGGTCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11224.13 Da
• Isoelectric Point: 10.0887

>AT150468 WP_012621770.1 NZ_CP049090:c1221602-1221306 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPNLVPVLQNL

Nucleotide

• Length: 297 bp

>AT150468 NZ_CP064686:c711542-711243 [Bradyrhizobium sp. 4]
ATGGCAAAAGTGAAATCATTCGATGCTGCGGAGTACCTCGACAGCCCCGAGATGATTGCGGCCTATCTCACCGAAGCCTT
TGAATCTGACGATCCGTCCGCAATAACGATGGCAATCGGTGCCGTAGCCCGTTCTCAGGGCATGGGCGCCATTGCCGAAA
AGGCCGGTCTATCCCGTGAGAACTTGTATCGCTCGCTTGGCGGCGAGGCCAAACCCGAATTCGGCACTGTGATAAAAGTT
CTCCACGCCATGGGTATCAATCTTGTGGCGCAACCCCAAAGTGGAACGAAGGCGGCGTAG

Structures

Toxin

• AlphaFold DB: U4SMB1