Detailed information of TA system TA150467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1122465..1123026
• Replicon: chromosome
• Accession: NZ_CP049090
• Organism: Glaesserella parasuis strain aHPS7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: G5S31_RS05470
• Protein ID: WP_005712614.1
• Coordinates: 1122465..1122743 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: G5S31_RS05475
• Protein ID: WP_005712616.1
• Coordinates: 1122754..1123026 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S31_RS05465	1121306..1122316	-	1011	WP_005712612.1	ornithine carbamoyltransferase	-
G5S31_RS05470	1122465..1122743	+	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G5S31_RS05475	1122754..1123026	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
G5S31_RS05480	1123090..1124310	+	1221	WP_160405614.1	ATP-dependent RNA helicase RhlB	-
G5S31_RS05485	1124320..1125807	+	1488	WP_160405613.1	DUF853 domain-containing protein	-
G5S31_RS05490	1125961..1126503	-	543	WP_021115958.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T150467 WP_005712614.1 NZ_CP049090:1122465-1122743 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T150467 NZ_CP064686:c711542-711243 [Bradyrhizobium sp. 4]
ATGGCAAAAGTGAAATCATTCGATGCTGCGGAGTACCTCGACAGCCCCGAGATGATTGCGGCCTATCTCACCGAAGCCTT
TGAATCTGACGATCCGTCCGCAATAACGATGGCAATCGGTGCCGTAGCCCGTTCTCAGGGCATGGGCGCCATTGCCGAAA
AGGCCGGTCTATCCCGTGAGAACTTGTATCGCTCGCTTGGCGGCGAGGCCAAACCCGAATTCGGCACTGTGATAAAAGTT
CTCCACGCCATGGGTATCAATCTTGTGGCGCAACCCCAAAGTGGAACGAAGGCGGCGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT150467 WP_005712616.1 NZ_CP049090:1122754-1123026 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT150467 NZ_CP064686:c711842-711546 [Bradyrhizobium sp. 4]
ATACCGACAATCAAGAGAACCGACGAATTCTCTGACTGGCTTAGAAAACAGCGAGACCGGAGAGCGAAGGCAAAAATCCT
CGCCCGGATCGATCGGCTGGCGCTCGGCAATCCCGGAGACGTTGCTGCGGTCGGAGGCGGCATCAGCGAAATGAGAGTCC
ATGAGGGCGCCGGATACCGGATCTACTACGTTGCCCGAGGCGAGGAGATCATCGTCCTTCTTTGCGGTGGCGATAAAGGC
TCTCAGCCGGGGGACATAGCGTTGGCAAAGAAGATCGCGAGCGAATTGGAGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A143CE91