Detailed information of TA system TA150454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 947380..948023
• Replicon: chromosome
• Accession: NZ_CP049089
• Organism: Glaesserella parasuis strain vHPS7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: G5C67_RS04850
• Protein ID: WP_005714197.1
• Coordinates: 947380..947724 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5C67_RS04855
• Protein ID: WP_016527809.1
• Coordinates: 947727..948023 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C67_RS04835	943206..943802	+	597	WP_021110885.1	DedA family protein	-
G5C67_RS04840	943985..945076	-	1092	WP_005714201.1	murein transglycosylase A	-
G5C67_RS04845	945186..947165	-	1980	WP_075630449.1	exoribonuclease II	-
G5C67_RS04850	947380..947724	+	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G5C67_RS04855	947727..948023	+	297	WP_016527809.1	helix-turn-helix domain-containing protein	Antitoxin
G5C67_RS04860	948086..949048	+	963	WP_021112452.1	WYL domain-containing protein	-
G5C67_RS04865	949629..950372	+	744	WP_021112448.1	protein phosphatase 2C domain-containing protein	-
G5C67_RS04870	950341..951093	+	753	WP_080652560.1	NAD-dependent protein deacylase	-
G5C67_RS04875	951170..952006	+	837	WP_075630450.1	NAD-dependent deacetylase	-
G5C67_RS04880	952033..952872	+	840	WP_075630451.1	NAD-dependent deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	945186..965453	20267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T150454 WP_005714197.1 NZ_CP049089:947380-947724 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T150454 NZ_CP064682:2759277-2760350 [Escherichia coli K-12]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11196.11 Da
• Isoelectric Point: 8.8246

>AT150454 WP_016527809.1 NZ_CP049089:947727-948023 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPELIPVLQGL

Nucleotide

• Length: 297 bp

>AT150454 NZ_CP064682:2760343-2760714 [Escherichia coli K-12]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• AlphaFold DB: U4SMB1