Detailed information of TA system TA150442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5045439..5046041
• Replicon: chromosome
• Accession: NZ_CP049085
• Organism: Escherichia coli strain p4A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: G6P91_RS24645
• Protein ID: WP_000897302.1
• Coordinates: 5045730..5046041 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6P91_RS24640
• Protein ID: WP_000356397.1
• Coordinates: 5045439..5045729 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P91_RS24610	5040746..5041675	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
G6P91_RS24615	5041857..5042099	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
G6P91_RS24620	5042389..5043237	+	849	WP_001038650.1	hypothetical protein	-
G6P91_RS24625	5043553..5044002	+	450	WP_032140890.1	hypothetical protein	-
G6P91_RS24630	5044187..5044405	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
G6P91_RS24635	5044802..5045080	-	279	WP_001296612.1	hypothetical protein	-
G6P91_RS24640	5045439..5045729	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G6P91_RS24645	5045730..5046041	-	312	WP_000897302.1	hypothetical protein	Toxin
G6P91_RS24650	5046270..5047178	+	909	WP_001385591.1	alpha/beta hydrolase	-
G6P91_RS24655	5047242..5048183	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G6P91_RS24660	5048228..5048665	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G6P91_RS24665	5048662..5049534	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G6P91_RS24670	5049528..5050127	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T150442 WP_000897302.1 NZ_CP049085:c5046041-5045730 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T150442 NZ_CP064682:c475764-475546 [Escherichia coli K-12]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT150442 WP_000356397.1 NZ_CP049085:c5045729-5045439 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT150442 NZ_CP064682:c476164-475790 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: S1P416