Detailed information of TA system TA150412

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105271..105629
• Replicon: plasmid p10A_p1
• Accession: NZ_CP049082
• Organism: Escherichia coli strain p10A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: G6P90_RS23690
• Protein ID: WP_012817833.1
• Coordinates: 105271..105429 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	105481..105629 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P90_RS23665	100831..101433	+	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
G6P90_RS23670	101730..102551	-	822	WP_001234469.1	DUF945 domain-containing protein	-
G6P90_RS23675	102670..102957	-	288	WP_000107535.1	hypothetical protein	-
G6P90_RS23680	103208..104212	+	1005	WP_024257664.1	IS110 family transposase	-
G6P90_RS23685	104375..104566	-	192	Protein_123	single-stranded DNA-binding protein	-
G6P90_RS23690	105271..105429	-	159	WP_012817833.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	105481..105629	-	149	NuclAT_0	-	Antitoxin
-	105481..105629	-	149	NuclAT_0	-	Antitoxin
-	105481..105629	-	149	NuclAT_0	-	Antitoxin
-	105481..105629	-	149	NuclAT_0	-	Antitoxin
G6P90_RS23695	105605..105880	-	276	WP_077631319.1	hypothetical protein	-
G6P90_RS23700	105944..107515	-	1572	WP_099588291.1	IS66 family transposase	-
G6P90_RS23705	107535..107882	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
G6P90_RS23710	107882..108529	-	648	WP_001295212.1	IS66 family insertion sequence hypothetical protein	-
G6P90_RS23715	108590..109366	+	777	WP_099588290.1	thiosulfate reductase cytochrome B subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / aph(3')-Ia / sul3 / ant(3'')-Ia / floR / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..143163	143163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6078.30 Da
• Isoelectric Point: 8.7390

>T150412 WP_012817833.1 NZ_CP049082:c105429-105271 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLMFTYLTRKSLCEIRYRDGDREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T150412 NZ_CP064681:c242726-242448 [Escherichia coli K-12]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 149 bp

>AT150412 NZ_CP049082:c105629-105481 [Escherichia coli]
CAGGGTGCAGACATATGGGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCATCCCATATGTCT

Structures

Antitoxin